Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
ShcA tyrosine phosphorylation sites can replace ShcA binding in signallingby middle T-antigen
Autore:
Nicholson, PR; Empereur, S; Glover, HR; Dilworth, SM;
Indirizzi:
Hammersmith Hosp, Imperial Coll Sch Med, Dept Metab Med, London W12 0NN, England Hammersmith Hosp London England W12 0NN tab Med, London W12 0NN, England
Titolo Testata:
EMBO JOURNAL
fascicolo: 22, volume: 20, anno: 2001,
pagine: 6337 - 6346
SICI:
0261-4189(20011115)20:22<6337:STPSCR>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACTIVATED PROTEIN-KINASE; EPIDERMAL GROWTH-FACTOR; ADAPTER PROTEIN; PHOSPHATIDYLINOSITOL 3-KINASE; GRB2-ASSOCIATED BINDER-1; FACTOR RECEPTOR; TUMOR-ANTIGEN; SH2 DOMAIN; GRB2; TRANSFORMATION;
Keywords:
polyoma virus MT; ShcA phosphorylation; signal transduction; transformation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
48
Recensione:
Indirizzi per estratti:
Indirizzo: Dilworth, SM Hammersmith Hosp, Imperial Coll Sch Med, Dept Metab Med, Du Cane Rd, London W12 0NN, England Hammersmith Hosp Du Cane Rd London EnglandW12 0NN , England
Citazione:
P.R. Nicholson et al., "ShcA tyrosine phosphorylation sites can replace ShcA binding in signallingby middle T-antigen", EMBO J, 20(22), 2001, pp. 6337-6346

Abstract

ShcA and Grb2 are crucial components in signalling by most tyrosine kinase-associated receptors. However, it is not clear whether Grb2 bound directlyto the receptor is equivalent to Grb2 associated via ShcA. We have used signalling stimulated by the middle T-antigen (MT) of polyoma virus to address this question. The two known Grb2-binding sites from murine ShcA, 313Y and 239/240YY, could functionally replace the NIT ShcA-interacting region in transformation assays using Rat2 fibroblasts. This demonstrates that signaloutput from membrane-bound ShcA requires only these two sequences and the ShcA-binding site in MT does not recruit other signalling molecules. Two standard Grb2-interacting sequences, either from the EGF receptor or the ShcA313Y region, could not replace the requirement for ShcA binding to MT, indicating an enhanced role for the ShcA 239/240YY motif. Sos1 and the dockingprotein Gab1 are brought into the MT complex through Grb2 association and this may be more effective using the 239/240YY sequence.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 19:31:23