Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Arrested differentiation and epithelial cell degeneration in zebrafish lens mutants
Autore:
Vihtelic, TS; Yamamoto, Y; Sweeney, MT; Jeffery, WR; Hyde, DR;
Indirizzi:
Univ Notre Dame, Dept Biol Sci, Notre Dame, IN 46556 USA Univ Notre Dame Notre Dame IN USA 46556 iol Sci, Notre Dame, IN 46556 USA Univ Notre Dame, Ctr Zebrafish Res, Notre Dame, IN 46556 USA Univ Notre Dame Notre Dame IN USA 46556 ish Res, Notre Dame, IN 46556 USA Univ Maryland, Dept Biol, College Pk, MD 20742 USA Univ Maryland College Pk MD USA 20742 Dept Biol, College Pk, MD 20742 USA
Titolo Testata:
DEVELOPMENTAL DYNAMICS
fascicolo: 4, volume: 222, anno: 2001,
pagine: 625 - 636
SICI:
1058-8388(200112)222:4<625:ADAECD>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
DANIO-RERIO; EYE DEVELOPMENT; TRANSCRIPTION FACTOR; HOMEOBOX GENE; C-MAF; EXPRESSION; MUTATIONS; MOUSE; RETINA; INDUCTION;
Keywords:
zebrafish; mutagenesis; ocular lens; cell differentiation; Prox1;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
48
Recensione:
Indirizzi per estratti:
Indirizzo: Hyde, DR Univ Notre Dame, Dept Biol Sci, POB 369, Notre Dame, IN 46556 USAUniv Notre Dame POB 369 Notre Dame IN USA 46556 ame, IN 46556 USA
Citazione:
T.S. Vihtelic et al., "Arrested differentiation and epithelial cell degeneration in zebrafish lens mutants", DEV DYNAM, 222(4), 2001, pp. 625-636

Abstract

In a chemical mutagenesis screen, we identified two zebrafish mutants thatpossessed small pupils. Genetic complementation revealed these two lines are due to mutations in different genes. The phenotypes of the two mutants were characterized using histologic, immunohistochemical, and tissue transplantation techniques. The arrested lens (arl) mutant exhibits a small eye and pupil phenotype at 48 hr postfertilization (hpf) and lacks any histologically identifiable lens structures by 5 days postfertilization (dpf). In contrast, the disrupted lens (dsl) mutants are phenotypically normal until 5 dpf, and then undergo lens disorganization and cell degeneration that is apparent by 7 dpf. Histology reveals the arl mutant terminates lens cell differentiation by 48 hpf, whereas the dsl lens exhibits a defective lens epithelial cell population at 5 dpf. Lens transplantation experiments demonstrateboth mutations are autonomous to the lens tissue. Immunohistochemistry reveals the retinal cells may suffer subtle effects, possibly due to the lens abnormalities. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 09:28:39