Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Disruption of erythropoiesis by dioxin in the zebrafish
Autore:
Belair, CD; Peterson, RE; Heideman, W;
Indirizzi:
Univ Wisconsin, Sch Pharm, Madison, WI 53705 USA Univ Wisconsin Madison WI USA 53705 sin, Sch Pharm, Madison, WI 53705 USA Univ Wisconsin, NIEHS, Dev & Mol Toxicol Ctr, Madison, WI USA Univ Wisconsin Madison WI USA HS, Dev & Mol Toxicol Ctr, Madison, WI USA
Titolo Testata:
DEVELOPMENTAL DYNAMICS
fascicolo: 4, volume: 222, anno: 2001,
pagine: 581 - 594
SICI:
1058-8388(200112)222:4<581:DOEBDI>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
ARYL-HYDROCARBON RECEPTOR; DIBENZO-P-DIOXINS; LIFE-STAGE DEVELOPMENT; DANIO-RERIO; LAKE TROUT; COMPARATIVE TOXICITY; GFP REPORTER; 2,3,7,8-TETRACHLORODIBENZO-P-DIOXIN; GENE; EXPRESSION;
Keywords:
TCDD; zebrafish; development; cardiovascular toxicity; hematopoiesis; erythropoiesis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Heideman, W Univ Wisconsin, Sch Pharm, 777 Highland Ave, Madison, WI 53705USA Univ Wisconsin 777 Highland Ave Madison WI USA 53705 53705 USA
Citazione:
C.D. Belair et al., "Disruption of erythropoiesis by dioxin in the zebrafish", DEV DYNAM, 222(4), 2001, pp. 581-594

Abstract

2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD, or dioxin) causes early life stage mortality in a variety of fish species. We have used the zebrafish (Danio rerio) to study the cardiovascular effects of TCDD treatment over the time course of zebrafish development. Early TCDD exposure (6 ng/ml) starting at 4 hr postfertilization (hpf) produced reductions in blood flow and in the number of circulating erythrocytes. These defects were consistently observable by 72 hpf. However, these responses were not observed when TCDD exposure was delayed until 96 hpf or later. These results suggest a model in which TCDD interferes with cardiovascular and erythropoietic developmental processes that are normally completed by 96 hpf. This model is strengthened bythe finding that TCDD exposure blocks the step in hematopoiesis in which developing zebrafish switch from the primitive phase to the definitive phaseof erythropoiesis. We observed no effect of TCDD on the levels of circulating primitive erythrocytes before 72 hpf and the expression of markers for early hematopoiesis, GATA-1 and GATA-2. However, early TCDD exposure prevented the appearance of definitive phase erythrocytes. TCDD produced a small delay in the migration of blood cells expressing SCL from the intermediate cell mass to the dorsal mesentery and dorsal aorta. Despite the decrease inblood flow produced by TCDD, confocal microscopy of the trunk vasculature by using a Tie2/green fluorescence protein endothelial marker at 48, 60, 72, and 96 hpf of TCDD-exposed (4 hpf) revealed no apparent defects in blood vessel structure. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/03/20 alle ore 19:21:35