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Titolo:
Microtubule patterning during meiotic maturation in mouse oocytes is determined by cell cycle-specific sorting and redistribution of gamma-tubulin
Autore:
Combelles, CMH; Albertini, DF;
Indirizzi:
Tufts Univ, Sch Med, Dept Anat & Cellular Biol, Boston, MA 02111 USA TuftsUniv Boston MA USA 02111 Anat & Cellular Biol, Boston, MA 02111 USA
Titolo Testata:
DEVELOPMENTAL BIOLOGY
fascicolo: 2, volume: 239, anno: 2001,
pagine: 281 - 294
SICI:
0012-1606(20011115)239:2<281:MPDMMI>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
ORGANIZING CENTERS; SPINDLE FORMATION; XENOPUS-OOCYTES; MITOTIC SPINDLE; PLASMA-MEMBRANE; NUCLEAR; CENTROSOME; FERTILIZATION; ORGANIZATION; CYTOSKELETON;
Keywords:
gamma-tubulin; centrosome; spindle; nuclear lamina; oocyte; meiosis; nuclear; cytoplasmic maturation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
59
Recensione:
Indirizzi per estratti:
Indirizzo: Albertini, DF Tufts Univ, Sch Med, Dept Anat & Cellular Biol, Boston, MA 02111 USA Tufts Univ Boston MA USA 02111 r Biol, Boston, MA 02111 USA
Citazione:
C.M.H. Combelles e D.F. Albertini, "Microtubule patterning during meiotic maturation in mouse oocytes is determined by cell cycle-specific sorting and redistribution of gamma-tubulin", DEVELOP BIO, 239(2), 2001, pp. 281-294

Abstract

The topography of microtubule assembly events during meiotic maturation ofanimal oocytes demands tight spatial control and temporal precision. To better understand what regulates the timing and location of microtubule assembly, synchronously maturing mouse oocytes were evaluated with respect to gamma -tubulin, pericentrin, and total tubulin polymer fractions at specific stages of meiotic progression. gamma -Tubulin remained associated with cytoplasmic centrosomes through diakinesis of meiosis-1. Following chromatin condensation and perinuclear centrosome aggregation, gamma -tubulin relocatedto a nuclear lamina-bounded compartment in which meiosis-1 spindle assembly occurred. gamma -Tubulin was stably associated with the meiotic spindle from prometaphase-1 through to anaphase-2, but also exhibited cell cycle-specific relocalization to cytoplasmic centrosomes. Specifically, anaphase onset of both meiosis-1 and -2 was characterized by the concomitant appearanceof gamma -tubulin and microtubule nucleation in subcortical centrosomes. Brief pulses of taxol applied at specific cell cycle stages enhanced detection of gamma -tubulin compartmentalization, consistent with a gamma -tubulinlocalization-dependent spatial restriction of microtubule assembly during meiotic progression. In addition, a taxol pulse during meiotic resumption impaired subsequent gamma -tubulin sorting, resulting in monopolar spindle formation and cell cycle arrest in meiosis-1; despite cell cycle arrest, polar body extrusion occurred roughly on schedule. Therefore, sorting of gamma-tubulin is involved in both the timing of location of meiotic spindle assembly as well as the coordination of karyokinesis and cytokinesis in mouse oocytes. (C) 2001 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 12:20:53