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Titolo:
Antimicrobial peptide defenses against pathogens associated with global amphibian declines
Autore:
Rollins-Smith, LA; Doersam, JK; Longcore, JE; Taylor, SK; Shamblin, JC; Carey, C; Zasloff, MA;
Indirizzi:
Vanderbilt Univ, Med Ctr, Dept Microbiol & Immunol, Nashville, TN 37232 USA Vanderbilt Univ Nashville TN USA 37232 & Immunol, Nashville, TN 37232 USA Vanderbilt Univ, Med Ctr, Dept Pediat, Nashville, TN 37232 USA Vanderbilt Univ Nashville TN USA 37232 pt Pediat, Nashville, TN 37232 USA Univ Maine, Dept Biol Sci, Orono, ME 04469 USA Univ Maine Orono ME USA 04469 v Maine, Dept Biol Sci, Orono, ME 04469 USA US EPA, Res Triangle Pk, NC 27111 USA US EPA Res Triangle Pk NC USA 27111US EPA, Res Triangle Pk, NC 27111 USA Univ Colorado, Dept Environm Populat & Organism Biol, Boulder, CO 80309 USA Univ Colorado Boulder CO USA 80309 & Organism Biol, Boulder, CO 80309 USA Magainin Pharmaceut Inc, Plymouth Meeting, PA 19462 USA Magainin Pharmaceut Inc Plymouth Meeting PA USA 19462 eting, PA 19462 USA
Titolo Testata:
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
fascicolo: 1, volume: 26, anno: 2002,
pagine: 63 - 72
SICI:
0145-305X(200201)26:1<63:APDAPA>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
POPULATION DECLINES; BASIDIOBOLUS-RANARUM; XENOPUS-LAEVIS; AEROMONAS-HYDROPHILA; MOLECULAR-CLONING; SKIN SECRETION; MASS MORTALITY; FROG; INFECTION; SEQUENCE;
Keywords:
amphibians; amphibian declines; antimicrobial peptides; Batrachochytrium; chytrid; frog; magainin; ranalexin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
56
Recensione:
Indirizzi per estratti:
Indirizzo: Rollins-Smith, LA Vanderbilt Univ, Med Ctr, Dept Microbiol & Immunol, Nashville, TN 37232 USA Vanderbilt Univ Nashville TN USA 37232 le, TN 37232 USA
Citazione:
L.A. Rollins-Smith et al., "Antimicrobial peptide defenses against pathogens associated with global amphibian declines", DEV COMP IM, 26(1), 2002, pp. 63-72

Abstract

Global declines of amphibian populations are a source of great concern. Several pathogens that can infect the skin have been implicated in the declines. The pathogen most frequently associated with recent die-offs is a chytrid fungus, Batrachochytrium dendrobatidis. A second fungus, Basidiobolus ranarum, was isolated from declining populations of Wyoming toads. A third pathogen, Aeromonas hydrophila, is an opportunistic bacterium found in healthy frogs, but capable of inducing disease. Among the immune defense mechanisms used by amphibians is the production of antimicrobial peptides in granular glands in the skin. These packets of natural antibiotics can be emptied onto the skin when the amphibian is injured. To determine whether antimicrobial skin peptides defend against these amphibian pathogens, six peptides (magainin I, magainin II, PGLa, CPF, ranalexin, and dermaseptin), from threespecies, and representing three structurally different families of peptides, were tested in growth inhibition assays. We show here that the peptides can kill or inhibit growth of both fungi but not Acromonas. Although each peptide varied in its effectiveness, at least one from each species was effective against both fungi at a concentration of about 10-20 muM. This is thefirst direct evidence that antimicrobial peptides in the skin can operate as a first line of defense against the organisms associated with global amphibian declines. It suggests that this innate defense mechanism may play a role in preventing or limiting infection by these organisms. (C) 2001 Elsevier Science Ltd. All rights reserved.

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Documento generato il 29/03/20 alle ore 18:06:02