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Titolo:
Trypanosoma cruzi is lysed by coelomic cytolytic factor-1, an invertebrateanalogue of tumor necrosis factor, and induces phenoloxidase activity in the coelomic fluid of Eisenia foetida foetida
Autore:
Fontt, EO; Beschin, A; Van Dijck, E; Vercruysse, V; Bilej, M; Lucas, R; De Baetselier, P; Vray, B;
Indirizzi:
Fac Med, Expt Immunol Lab, B-1070 Brussels, Belgium Fac Med Brussels Belgium B-1070 pt Immunol Lab, B-1070 Brussels, Belgium Free Univ Brussels, Flemish Interuniv Inst Biotechnol, Dept Immunol Parasitol & Ultrastruct, Brussels, Belgium Free Univ Brussels Brussels Belgiumol & Ultrastruct, Brussels, Belgium Acad Sci Czech Republ, Inst Microbiol, Dept Immunol & Gnotobiol, Prague, Czech Republic Acad Sci Czech Republ Prague Czech Republic iol, Prague, Czech Republic
Titolo Testata:
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
fascicolo: 1, volume: 26, anno: 2002,
pagine: 27 - 34
SICI:
0145-305X(200201)26:1<27:TCILBC>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
EXPERIMENTAL AFRICAN TRYPANOSOMIASIS; PROPHENOLOXIDASE-ACTIVATING SYSTEM; CRAYFISH PACIFASTACUS-LENIUSCULUS; FACTOR-ALPHA; DEPENDENT MECHANISM; PROTECTIVE ROLE; CHAGAS-DISEASE; NITRIC-OXIDE; BRUCEI; MACROPHAGES;
Keywords:
coelomic cytotoxic factor; tumor necrosis factor; CCF-1; innate immunity; lectin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
47
Recensione:
Indirizzi per estratti:
Indirizzo: Vray, B Fac Med, Expt Immunol Lab, CP 615,808 Route de Lennik, B-1070 Brussels, Belgium Fac Med CP 615,808 Route de Lennik Brussels Belgium B-1070 elgium
Citazione:
E.O. Fontt et al., "Trypanosoma cruzi is lysed by coelomic cytolytic factor-1, an invertebrateanalogue of tumor necrosis factor, and induces phenoloxidase activity in the coelomic fluid of Eisenia foetida foetida", DEV COMP IM, 26(1), 2002, pp. 27-34

Abstract

A cytolytic protein named Coelomic Cytolytic Factor-1 (CCF-1) was isolatedfrom the coelomic fluid of the earthworm Eisenia foetida foetidi. Despite the absence of any gene homology, CCF-1 showed functional analogy with the mammalian cytokine tumour necrosis factor (TNF), particularly based on similar lectin-like activity. Indeed, both CCF-1 and TNF recognise N,N'-diacetylchitobiose and exert lytic activity on African Trypanosoma brucei brucei. In this report, we show that South-American Trypanosoma cruzi trypomastigotes, but not epimastigotes, were lysed by earthworm coelomic fluid or purified CCF-1. However, T. cruzi was less susceptible to lysis than T brucei brucei. This lytic effect of coelomic fluid and CCF-1 on T. eruzi trypomastigotes was partially inhibited in the presence of anti-CCF-1 monoclonal antibody, antibody neutralising the lectin-like activity of TNF or N,N'-diacetylchitobiose. In contrast, this lytic effect was completely inhibited when using T b. brucei. In addition, T. cruzi components, upon recognition by CCF-1in E.f. foetida coelomic fluid, triggered the prophenoloxidase cascade, aninvertebrate defence mechanism. These results further extend the functional analogies of CCF-1 and TNF, suggesting that both molecules share a similar lectin-like activity that has been conserved as an innate recognition mechanism in invertebrates and vertebrates. They also establish a link betweenstercorarian (T. cruzi) and salivarian (T. brucei) trypanosomatids having divergent phylogenetic origins and patterns of evolution, but possessing closely related cell surface sugar moieties. (C) 2001 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/11/20 alle ore 00:30:20