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Titolo:
A yeast homolog of chromatin assembly factor 1 is involved in early ribosome assembly
Autore:
Schaper, S; Fromont-Racine, M; Linder, P; de la Cruz, J; Namane, A; Yaniv, M;
Indirizzi:
Inst Pasteur, Dept Biochim & Genet Mol, Lab Chim Struct Macromol, URA 2185CNRS, F-75724 Paris 15, France Inst Pasteur Paris France 15 mol, URA 2185CNRS, F-75724 Paris 15, France Univ Geneva, Ctr Med Univ, Dept Biochim Med, CH-2111 Geneva, Switzerland Univ Geneva Geneva Switzerland CH-2111 Med, CH-2111 Geneva, Switzerland Inst Pasteur, Dept Biotechnol, Unite Genet Interact Macromol, F-75724 Paris 15, France Inst Pasteur Paris France 15 Interact Macromol, F-75724 Paris 15, France Inst Pasteur, Dept Biotechnol, URA 1644 CNRS, Unite Virus Oncogenes, F-75724 Paris 15, France Inst Pasteur Paris France 15 e Virus Oncogenes, F-75724 Paris 15, France
Titolo Testata:
CURRENT BIOLOGY
fascicolo: 23, volume: 11, anno: 2001,
pagine: 1885 - 1890
SICI:
0960-9822(20011127)11:23<1885:AYHOCA>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANS-ACTING FACTORS; SACCHAROMYCES-CEREVISIAE; SUBUNITS; PROTEIN; RNA; POLYRIBOSOMES; PURIFICATION; COMPLEX; L25;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
18
Recensione:
Indirizzi per estratti:
Indirizzo: Schaper, S Max Planck Inst Mol Genet, Otto Warburg Labs, Ihnestr 73, D-14195 Berlin, Germany Max Planck Inst Mol Genet Ihnestr 73 Berlin Germany D-14195 ny
Citazione:
S. Schaper et al., "A yeast homolog of chromatin assembly factor 1 is involved in early ribosome assembly", CURR BIOL, 11(23), 2001, pp. 1885-1890

Abstract

Cells have a recurrent need for the correct assembly of protein-nucleic acid complexes. We have studied a yeast homolog of the smallest subunit of chromatin assembly factor 1 (CAM), encoded by YMR131c and termed "RRB1" [1]. Unlike other yeast homologs, Msi1p, and Hat2p, Rrb1p is essential for cell viability. Impairment of Rrb1p function results in decreased levels of free60S ribosomal subunits and the appearance of half-mer polysomes, suggesting its involvement in ribosome biogenesis. Using tandem affinity purification (TAP [2]) combined with mass spectrometry, we show that Rrb1p is associated with ribosomal protein L3. A fraction of Rrb1p is also found in a protein-precursor rRNA complex containing at least ten other early-assembling ribosomal proteins. We propose that Rrb1p is required for proper assembly of preribosomal particles during early ribosome biogenesis, presumably by targeting L3 onto the 35S precursor rRNA. This action may resemble the mechanismby which CAM assembles histones H3/H4 onto newly replicated DNA.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 16:13:49