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Titolo:
IgE binding to soluble and insoluble wheat flour proteins in atopic and non-atopic patients suffering from gastrointestinal symptoms after wheat ingestion
Autore:
Simonato, B; De Lazzari, F; Pasini, G; Polato, F; Giannattasio, M; Gemignani, C; Peruffo, ADB; Santucci, B; Plebani, M; Curioni, A;
Indirizzi:
Univ Padua, Dipartimento Biotecnol Agr, Padua, Italy Univ Padua Padua Italy Padua, Dipartimento Biotecnol Agr, Padua, Italy Univ Padua, Gastroenterol Dipartimento Sci Chirurg & Gastroen, Padua, Italy Univ Padua Padua Italy ipartimento Sci Chirurg & Gastroen, Padua, Italy Univ Naples Federico II, Dipartimento Arboricoltura Bot & Patol Vegetale, Naples, Italy Univ Naples Federico II Naples Italy ot & Patol Vegetale, Naples, Italy Univ Padua, Dipartimento Med Ambientale & Sanit Pubbl Med Lav, Padua, Italy Univ Padua Padua Italy d Ambientale & Sanit Pubbl Med Lav, Padua, Italy Univ Verona, Dipartimento Sci & Tecnol, Verona, Italy Univ Verona VeronaItaly rona, Dipartimento Sci & Tecnol, Verona, Italy IRCSS San Gallicano, Rome, Italy IRCSS San Gallicano Rome ItalyIRCSS San Gallicano, Rome, Italy Azienda Osped Padova, Dipartimento Med Lab, Padua, Italy Azienda Osped Padova Padua Italy va, Dipartimento Med Lab, Padua, Italy
Titolo Testata:
CLINICAL AND EXPERIMENTAL ALLERGY
fascicolo: 11, volume: 31, anno: 2001,
pagine: 1771 - 1778
SICI:
0954-7894(200111)31:11<1771:IBTSAI>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
IRRITABLE BOWEL SYNDROME; ALPHA-AMYLASE-INHIBITOR; BAKERS ASTHMA; FOOD ALLERGY; DOUBLE-BLIND; HYPERSENSITIVITY; IDENTIFICATION; DERMATITIS; BARLEY; SUBUNITS;
Keywords:
atopy; food allergy; irritable bowel syndrome; wheat;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Curioni, A Fac Sci, Dipartimento Sci & Tecnol, Strada Grazie 15, I-37134 Verona, Italy Fac Sci Strada Grazie 15 Verona Italy I-37134 34 Verona, Italy
Citazione:
B. Simonato et al., "IgE binding to soluble and insoluble wheat flour proteins in atopic and non-atopic patients suffering from gastrointestinal symptoms after wheat ingestion", CLIN EXP AL, 31(11), 2001, pp. 1771-1778

Abstract

Background The involvement of IgE-mediated hypersensitivity reactions in the genesis of gastrointestinal symptoms after ingestion of foods containingwheat has been rarely reported. Objective Ta detect IgE specifically binding to wheat proteins in the. sera of atopic and non-atopic patients suffering from gastrointestinal symptoms after ingestion of wheat and to evaluate the reliability of skin prick test and CAP in the diagnosis of food allergy to wheat. Methods The sera of patients (10 atopic and 10 non-atopic) previously diagnosed as suffering from irritable bowel syndrome and complaining of symptoms after wheat ingestion were analysed by immunoblotting for IgE binding to water/salt-soluble and insoluble wheat flour proteins. Results All the atopic patients and only one of the non-atopic patients were positive to wheat CAP. For the patients tested, skin prick test was positive for all the atopic patients and for only one of the non-atopic patients. However, immunoblotting experiments showed the presence of specific IgE to wheat proteins in all the patients. Ten out of 11 of the wheat CAP-positive patients had IgE binding to a soluble 16-kDa band, but the same band was recognized, in a slighter way, by only two out of nine of the wheat CAP-negative patients. Moreover, although almost all of the patients were negative in CAP testing with gluten, 19 out of 20 recognized protein bands belonging to the prolamin fraction. Conclusions For the atopic patients the positivity to skin prick test and CAP to wheat was in accordance with the immunoblotting results and a food allergy to wheat could be diagnosed. In these patients a major allergen was a 16-kDa band corresponding to members of the cereal alpha -amylase/trypsininhibitors protein family, the major allergens involved in baker's asthma. In the non-atopic patients the positive immunoblotting results contrasted with the responses of the allergologic tests, indicating that the allergenic wheat protein preparations currently used are of limited value in detecting specific IgE to wheat and that the fraction of irritable bowel syndrome (IBS) patients vith food allergy may be larger than believed.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 09:47:03