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Titolo:
Sputum induction: effect of nebulizer output and inhalation tine on cell counts and fluid-phase measures
Autore:
Belda, J; Hussack, P; Dolovich, M; Efthimiadis, A; Hargreave, FE;
Indirizzi:
St Josephs Healthcare, Dept Med, Asthma Res Grp, Hamilton, ON L8N 4A6, Canada St Josephs Healthcare Hamilton ON Canada L8N 4A6 lton, ON L8N 4A6, Canada McMaster Univ, Hamilton, ON, Canada McMaster Univ Hamilton ON CanadaMcMaster Univ, Hamilton, ON, Canada
Titolo Testata:
CLINICAL AND EXPERIMENTAL ALLERGY
fascicolo: 11, volume: 31, anno: 2001,
pagine: 1740 - 1744
SICI:
0954-7894(200111)31:11<1740:SIEONO>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
AIRWAY INFLAMMATION; HEALTHY-SUBJECTS; INDEXES; ASTHMA; SAMPLES;
Keywords:
induced sputum; sputum cell counts; sputum chemistry;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
16
Recensione:
Indirizzi per estratti:
Indirizzo: Hargreave, FE St Josephs Healthcare, Firestone Inst Resp Hlth, Hamilton, ON L8N 4A6, Canada St Josephs Healthcare Hamilton ON Canada L8N 4A6 A6, Canada
Citazione:
J. Belda et al., "Sputum induction: effect of nebulizer output and inhalation tine on cell counts and fluid-phase measures", CLIN EXP AL, 31(11), 2001, pp. 1740-1744

Abstract

Background A knowledge of the factors that can affect induced sputum results is essential in order to standardize the procedure. Objective We investigated the influence of nebulizer output on sputum cellcounts and fluid phase measurements at increasing times of sputum induction. Methods Eighteen adults with stable asthma inhaled an aerosol of 3% hypertonic saline to induce sputum after 7, 14 and 21 min on 2 days separated by 48 h. On one day, in random order, the ultrasonic nebulizer used had a relatively low output of 0.87 mL/min (particle size 5.58 mum mass median aerodynamic diameter, MMAD) and, on the other, a higher output of 1.90 mL/min (particle size 4.14 mum MMAD). The sputum was selected from each expectorate and examined blind to the induction procedures. Results With both nebulizers, the 14- and 21-min samples were lower in weight, neutrophils, eosinophils, eosinophil cationic protein (ECP) and interleukin (IL)-8 and higher in macrophages. The higher output nebulizer inducedsputum with higher cell viability and lower ECP and IL-8. Conclusion The results identify that the volume of hypertonic saline inhaled in sputum induction influences the fluid-phase measurements. The duration of induction does alter the cell counts and suggests that the later expectorated sputum samples originate from more peripheral airways. The results draw attention to the need to standardize the volume and time of nebulization to accurately interpret and compare results.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/01/20 alle ore 13:13:16