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OPAC HELP

Titolo:
Piceatannol is an effective inhibitor of IgE-mediated secretion from humanbasophils but is neither selective for this receptor nor acts on syk kinase at concentrations where mediator release inhibition occurs
Autore:
Miura, K; Lavens-Phillips, S; MacGlashan, DW;
Indirizzi:
Johns Hopkins Asthma & Allergy Ctr, Baltimore, MD 21224 USA Johns Hopkins Asthma & Allergy Ctr Baltimore MD USA 21224 e, MD 21224 USA
Titolo Testata:
CLINICAL AND EXPERIMENTAL ALLERGY
fascicolo: 11, volume: 31, anno: 2001,
pagine: 1732 - 1739
SICI:
0954-7894(200111)31:11<1732:PIAEIO>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
FC-EPSILON-RI; IMMUNOGLOBULIN-E-RECEPTOR; PROTEIN-TYROSINE KINASE; HIGH-AFFINITY RECEPTOR; MAST-CELLS; ACTIVATION MOTIFS; LYN; PHOSPHORYLATION; STIMULATION; HISTAMINE;
Keywords:
human basophils; syk kinase; piceatannol; histamine release; LTC4 release; shc; calcium; anti-IgE; formyl-met-leu-phe; phorbol ester;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: MacGlashan, DW Johns Hopkins Asthma & Allergy Ctr, 5501 Hopkins Bayview Circle, Baltimore, MD 21224 USA Johns Hopkins Asthma & Allergy Ctr 5501 Hopkins Bayview Circle Baltimore MD USA 21224
Citazione:
K. Miura et al., "Piceatannol is an effective inhibitor of IgE-mediated secretion from humanbasophils but is neither selective for this receptor nor acts on syk kinase at concentrations where mediator release inhibition occurs", CLIN EXP AL, 31(11), 2001, pp. 1732-1739

Abstract

Background Syk kinase is probably an early necessary tyrosine kinase involved in IgE-mediated secretion from human basophils. Causal testing of the role of syk kinase in the secretion requires a selective pharmacological agent. Piceatannol has previously been used to demonstrate the causal role of syk in secretion but its selectively has recently come into question. Objective To determine whether piceatannol inhibits IgE-mediated signalling events in a manner consistent with its putative inhibitory effects on sykkinase and at concentrations relevant to its inhibition of mediator release. Methods Human basophils were examined for the effects of piceatannol on mediator release or various signalling steps. Results We show that while piceatannol has an IC50 for inhibition of IgE-mediated histamine release of 3-5 muM, these same concentrations inhibit secretion of phorbol 12-myristate 13-acetate (PMA)-induced histamine release (as previously shown) and leukotriene C (LTC)4 release induced by fMLP. Concentrations of piceatannol up to 100 muM also did not inhibit IgE-mediated phosphorylation of shc, a immediate downstream target of syk kinase. Similarconcentrations also did not inhibit IgE-mediated cytosolic calcium elevations, another downstream signal thought to be dependent on syk kinase. In contrast, piceatannol did modify the cytosolic calcium response that follows stimulation with formyl methionyl-leucyl-phenylalanine (fMLP). Conclusion Taken together with published studies using other cell types, we conclude that piceatannol does not inhibit secretion from human basophilsby inhibiting the activity of syk kinase.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/01/20 alle ore 21:02:18