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Titolo:
Development of a high resolution UV spectrophotometer for at-line monitoring of bioprocesses
Autore:
Noui, L; Hill, J; Keay, PJ; Wang, RY; Smith, T; Yeung, K; Habib, G; Hoare, M;
Indirizzi:
Univ Luton, Fac Sci Technol & Design, Luton LU1 5DU, Beds, England Univ Luton Luton Beds England LU1 5DU esign, Luton LU1 5DU, Beds, England Arun Technol Ltd, Southwater RH13 7UD, W Sussex, England Arun Technol LtdSouthwater W Sussex England RH13 7UD , W Sussex, England Univ London Univ Coll, Dept Biochem Engn, Adv Ctr Biochem Engn, London WC1E 7JE, England Univ London Univ Coll London England WC1E 7JE , London WC1E 7JE, England
Titolo Testata:
CHEMICAL ENGINEERING AND PROCESSING
fascicolo: 2, volume: 41, anno: 2002,
pagine: 107 - 114
SICI:
0255-2701(200202)41:2<107:DOAHRU>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
NUCLEIC-ACIDS;
Keywords:
CCD detector; downstream processing; bioprocess monitoring; partial least squares; yeast; protein;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Engineering, Computing & Technology
Citazioni:
19
Recensione:
Indirizzi per estratti:
Indirizzo: Wang, RY Univ Luton, Fac Sci Technol & Design, 2 Adelaide St, Luton LU1 5DU, Beds, England Univ Luton 2 Adelaide St Luton Beds England LU1 5DU Beds, England
Citazione:
L. Noui et al., "Development of a high resolution UV spectrophotometer for at-line monitoring of bioprocesses", CHEM ENG P, 41(2), 2002, pp. 107-114

Abstract

The construction, calibration and application of a direct readout UV spectrophotometer are described. The instrument utilises a concave holographic flat field diffraction grating and a charge coupled device (CCD) as a detector which measures a continuous spectrum, allowing several analytes to be monitored simultaneously. The advantages over conventional spectrophotometerscome from miniaturisation and the near-instantaneous capture of full spectra. The spectrophotometer was characterised in terms of spectral range (185.0-380.0 nm), spectral resolution (0.3 nm with 10 mum slit width), absorbance precision (standard deviation (STD): +/- 0.0009), dynamic range (0.00 to2.00 absorbance unit (AU)), and accuracy (0.5771 AU with 0.0012 STD at 313.0 nm). The efficiency and reliability of the instrument were demonstrated using artificial mixtures of bovine serum albumin (BSA), ribonucleic acid (RNA) and cell debris of defined composition to mimic Saccharomyces cerevisiae homogenate, as well as real Saccharomyces cerevisiae homogenates. The application of the spectrophotometer in conjunction with the partial least squares (PLS) technique in at-line monitoring of selective flocculation processes purification of Saccharomyces cerevisiae homogenates have shown good agreement with the results obtained from off-line chemical assays. (C) 2002 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 13/07/20 alle ore 03:50:11