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Titolo:
Expression of inducible Hsp70 enhances the proliferation of MCF-7 breast cancer cells and protects against the cytotoxic effects of hyperthermia
Autore:
Barnes, JA; Dix, DJ; Collins, BW; Luft, C; Allen, JW;
Indirizzi:
US EPA, Natl Res Council, Natl Hlth & Environm Effects Res Lab, Off Res & Dev, Res Triangle Pk, NC 27711 USA US EPA Res Triangle Pk NC USA 27711 & Dev, Res Triangle Pk, NC 27711 USA US EPA, Div Environm Carcinogenesis, Natl Hlth & Environm Effects Res Lab,Off Res & Dev, Res Triangle Pk, NC 27711 USA US EPA Res Triangle Pk NC USA27711 & Dev, Res Triangle Pk, NC 27711 USA US EPA, Reprod Toxicol Div, Natl Hlth & Environm Effects Res Lab, Off Res & Dev, Res Triangle Pk, NC 27711 USA US EPA Res Triangle Pk NC USA 27711 & Dev, Res Triangle Pk, NC 27711 USA
Titolo Testata:
CELL STRESS & CHAPERONES
fascicolo: 4, volume: 6, anno: 2001,
pagine: 316 - 325
SICI:
1355-8145(200110)6:4<316:EOIHET>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEAT-SHOCK PROTEINS; C6 GLIOMA-CELLS; GENE-EXPRESSION; ESTROGEN-RECEPTOR; MOLECULAR CHAPERONES; MAMMALIAN-CELLS; MYC PROTEIN; IN-VIVO; THERMOTOLERANCE; P53;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
50
Recensione:
Indirizzi per estratti:
Indirizzo: Barnes, JA US EPA, Natl Res Council, Natl Hlth & Environm Effects Res Lab,Off Res & Dev, Res Triangle Pk, NC 27711 USA US EPA Res Triangle Pk NC USA 27711 Triangle Pk, NC 27711 USA
Citazione:
J.A. Barnes et al., "Expression of inducible Hsp70 enhances the proliferation of MCF-7 breast cancer cells and protects against the cytotoxic effects of hyperthermia", CELL STR CH, 6(4), 2001, pp. 316-325

Abstract

Heat shock proteins (Hsps) are ubiquitous proteins that are induced following exposure to sublethal heat shock, are highly conserved during evolution, and protect cells from damage through their function as molecular chaperones. Some cancers demonstrate elevated levels of Hsp70, and their expression has been associated with cell proliferation, disease prognosis, and resistance to chemotherapy. In this study, we developed a tetracycline-regulatedgene expression system to determine the specific effects of inducible Hsp70 on cell growth and protection against hyperthermia in MCF-7 breast cancercells. MCF-7 cells expressing high levels of Hsp70 demonstrated a significantly faster doubling time (39 hours) compared with nonoverexpressing control cells (54 hours). The effect of elevated Hsp70 on cell proliferation wascharacterized further by 5-bromo-2'deoxyuridine labeling, which demonstrated a higher number of second and third division metaphases in cells at 42 and 69 hours, respectively. Estimates based on cell cycle analysis and mean doubling time indicated that Hsp70 may be exerting its growth-stimulating effect on MCF-7 cells primarily by shortening of the G(0)/G(1) and S phases of the cell cycle. In addition to the effects on cell growth, we found thatelevated levels of Hsp70 were sufficient to confer a significant level of protection against heat in MCF-7 cells. The results of this study support existing evidence linking Hsp70 expression with cell growth and cytoprotection in human cancer cells.

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Documento generato il 15/01/21 alle ore 22:51:39