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Titolo:
Down-regulation of T-STAR, a growth inhibitory protein, after SV40-mediated immortalization
Autore:
Kool, J; van Zaane, W; van der Eb, AJ; Terleth, C;
Indirizzi:
Leiden Univ, Ctr Med, Dept Radiat Genet & Chem Mutagenesis, Sylvius Lab, NL-2333 AL Leiden, Netherlands Leiden Univ Leiden Netherlands NL-2333 AL NL-2333 AL Leiden, Netherlands
Titolo Testata:
CELL GROWTH & DIFFERENTIATION
fascicolo: 11, volume: 12, anno: 2001,
pagine: 535 - 541
SICI:
1044-9523(200111)12:11<535:DOTAGI>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
NUCLEAR-LOCALIZATION SIGNAL; DIFFERENTIAL DISPLAY; CELLULAR SENESCENCE; HUMAN-FIBROBLASTS; HUMAN-CELLS; TELOMERASE; SAM68; RNA; IDENTIFICATION; ACTIVATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Terleth, C Leiden Univ, Ctr Med, Dept Radiat Genet & Chem Mutagenesis, Sylvius Lab, Wassenaarseweg 72, NL-2333 AL Leiden, Netherlands Leiden Univ Wassenaarseweg 72 Leiden Netherlands NL-2333 AL ds
Citazione:
J. Kool et al., "Down-regulation of T-STAR, a growth inhibitory protein, after SV40-mediated immortalization", CELL GROWTH, 12(11), 2001, pp. 535-541

Abstract

Normal human cells can undergo a limited number of divisions, whereas transformed cells may have an extended life span and can give rise to immortal cells. To isolate genes involved in the immortalization process, gene expression in SV40-transformed preimmortal human fibroblasts was compared with expression in SV40-transformed immortalized fibroblasts using an mRNA differential display. We found that the growth-inhibitory protein testis-signal transduction and activation of RNA (T-STAR) a homologue of cell-cycle regulator Sam68, is strongly down-regulated in immortalized cells. Overexpressionof T-STAR in the SV40-transformed immortalized cells resulted in a strong reduction of colony formation, whereas deletion of the RNA-binding domain of T-STAR abrogated this effect. Down-regulation of testis-signal transduction and activation of RNA (T-STAR) expression is found only in immortal cells isolated after a proliferative crisis accompanied with massive cell death. The strict correlation of down-regulation of T-STAR expression only in those immortal cells that arose after a clear proliferative crisis suggests that the loss of T-STAR might be, necessary to bypass crisis.

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Documento generato il 21/09/20 alle ore 12:28:16