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Titolo:
Could plasmid-mediated gene transfer into the myocardiurn be augmented by left ventricular guided laser myocardial injury?
Autore:
Fuchs, S; Baffour, R; Shou, M; Stabile, E; Singh, S; Schwartz, B; Branellec, D; Epstein, SE; Kornowski, R;
Indirizzi:
Cardiovasc Res Inst, Washington, DC 20010 USA Cardiovasc Res Inst Washington DC USA 20010 nst, Washington, DC 20010 USA Aventis Gencell, Vitry Sur Seine, France Aventis Gencell Vitry Sur SeineFrance Gencell, Vitry Sur Seine, France
Titolo Testata:
CATHETERIZATION AND CARDIOVASCULAR INTERVENTIONS
fascicolo: 4, volume: 54, anno: 2001,
pagine: 533 - 538
SICI:
1522-1946(200112)54:4<533:CPGTIT>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
PORCINE HEARTS; ANGIOGENESIS; INJECTION; THERAPY; REVASCULARIZATION; ELECTROPORATION; VECTORS; DISEASE; SAFETY; SYSTEM;
Keywords:
myocardium; plasmid; gene therapy; laser;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Fuchs, S Cardiovasc Res Inst, 110 Irving St,NW,Suite 4B-1, Washington, DC 20010 USA Cardiovasc Res Inst 110 Irving St,NW,Suite 4B-1 Washington DC USA20010
Citazione:
S. Fuchs et al., "Could plasmid-mediated gene transfer into the myocardiurn be augmented by left ventricular guided laser myocardial injury?", CATHET C IN, 54(4), 2001, pp. 533-538

Abstract

Early studies have indicated no correlation between the amount of mechanical injury and the level of myocardial gene expression following direct plasmid vector injection. Recently, however, evidence suggests that combined laser myocardial injury and plasmid-based gene delivery exert synergistic effects on gene expression and activity. The purpose of the study was to determine whether laser-induced myocardial injury followed by transendocardial gene transfer increases gene expression compared to gene transfer alone. We assessed the ability of a plasmid vector to express its transgene after injection into porcine ischemic myocardium with and without preceding laser myocardial injury. Thirteen animals had transendocardial injections of the luciferase reporter gene in a plamid vector using a catheter-based injection system. Injections (0.5 mg per animal, 50 mug per injection site) were divided into 10 sites in the ischemic territory. Eight animals underwent transendocardial laser injury of the ischemic region (2 Joule per pulse x 10 sites) prior to gene delivery. In five animals, gene injection sites were dispersed between laser channels, and in three animals laser and gene delivery were applied in close proximity (< 5 mm) or at the same location. Luciferaseactivity was measured at 3 and 7 days. Luciferase expression in ischemic zones was markedly elevated at day 3 and 7, and similar whether animals werepretreated using laser injury followed by gene transfer compared to gene transfer alone. Neither same-spot injection nor dispersed gene delivery wereassociated with augmented gene expression compared to gene transfer alone. Using the above-described catheter-based approach to combine localized laser injury and injection of naked DNA into ischemic myocardium, laser injurydid not augment gene expression above levels present with gene transfer alone. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/20 alle ore 12:52:09