Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Orexin A and B evoke noradrenaline release from rat cerebrocortical slices
Autore:
Hirota, K; Kushikata, T; Kudo, M; Kudo, T; Lambert, DG; Matsuki, A;
Indirizzi:
Hirosaki Univ, Sch Med, Dept Anesthesiol, Hirosaki, Aomori 0368563, Japan Hirosaki Univ Hirosaki Aomori Japan 0368563 rosaki, Aomori 0368563, Japan Leicester Royal Infirm, Dept Anaesthesia Crit Care & Pain Management, Leicester LE1 5WW, Leics, England Leicester Royal Infirm Leicester Leics England LE1 5WW WW, Leics, England
Titolo Testata:
BRITISH JOURNAL OF PHARMACOLOGY
fascicolo: 7, volume: 134, anno: 2001,
pagine: 1461 - 1466
SICI:
0007-1188(200112)134:7<1461:OAABEN>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
SLEEP-WAKEFULNESS; BRAIN; NEUROENDOCRINE; NOREPINEPHRINE; INVOLVEMENT; NARCOLEPSY; RECEPTORS; MEDULLA; SYSTEMS; NEURONS;
Keywords:
orexin; noradrenaline; rat cerebrocortical slices;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Hirota, K Hirosaki Univ, Sch Med, Dept Anesthesiol, Hirosaki, Aomori 0368563, Japan Hirosaki Univ Hirosaki Aomori Japan 0368563 mori 0368563, Japan
Citazione:
K. Hirota et al., "Orexin A and B evoke noradrenaline release from rat cerebrocortical slices", BR J PHARM, 134(7), 2001, pp. 1461-1466

Abstract

1 Orexin A and B, recently identified in the rat hypothalamus are endogenous neuropeptide agonists for the G-protein coupled orexin-1 (OX1) and orexin-2 (OX2) receptors.2 In the present study, we have examined the effects of orexin A, B and raised extracellular K+ on noradrenaline release from the rat cerebrocorticalslice. We have compared this with other sleep-wake-related (excitatory) neurotransmitters; dopamine, glutamate, serotonin and histamine.3 Neurotransmitter release studies were performed in rat cerebrocortical slices incubated in modified Krebs buffer (with and without Ca2+ +EGTA 1 mM)with various concentrations of orexin A, B and K+ for various times.4 Orexin A and 13-evoked (10(-7) M) noradrenaline release was time-dependent reaching a maximum some 10 min after stimulation. K+ (40 mM) evoked release was also time dependent but reached a maximum after 6 min. Orexin A, B and K+ stimulation of release was concentration dependent with pEC(50) and E-max (% of basal) values of 8.74+/-0.32 (1.8 nM) and 263+/-14% and 8.61+/-0.38 (2.4 nM) and 173+/-7% and 1.43+/-0.02 (37 mM) and 1430+/-70%, respectively. Orexin-evoked release was partially extracellular Ca2+ dependent.5 Of the other transmitters studied there was a weak orexin A and B stimulation of glutamate release. In contrast K+ evoked dopamine, glutamate, histamine and serotonin release with pEC(50) and E-max (% of basal) values of 1.47+/-0.05 (34 mM) and 3430+/-410%, 1.38+/-0.04 (42 mM) and 1240+/-50%, 1.47+/-0.02 (34 mM) and 480+/-10% and 1.40+/-0.05 (40 mM) and 560+/-60% respectively.6 We conclude that the neuropeptides orexin A and B evoke noradrenaline release from rat cerebrocortical slices.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 14/08/20 alle ore 04:57:41