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Titolo:
Production of ordered collagen matrices for three-dimensional cell culture
Autore:
Besseau, L; Coulomb, B; Lebreton-Decoster, C; Giraud-Guille, MM;
Indirizzi:
Univ Paris 06, Observ Oceanol, F-66650 Banyuls sur Mer, France Univ Paris 06 Banyuls sur Mer France F-66650 650 Banyuls sur Mer, France CNRS, UMR 7628, F-66650 Banyuls sur Mer, France CNRS Banyuls sur Mer France F-66650 628, F-66650 Banyuls sur Mer, France Hop St Louis, INSERM, U532, Inst Rech Peau, F-75475 Paris, France Hop St Louis Paris France F-75475 Inst Rech Peau, F-75475 Paris, France Univ Paris 06, Ecole Prat Hautes Etud, F-75005 Paris, France Univ Paris 06 Paris France F-75005 at Hautes Etud, F-75005 Paris, France CNRS, F-75005 Paris, France CNRS Paris France F-75005CNRS, F-75005 Paris, France
Titolo Testata:
BIOMATERIALS
fascicolo: 1, volume: 23, anno: 2002,
pagine: 27 - 36
SICI:
0142-9612(200201)23:1<27:POOCMF>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN-FIBROBLASTS; DERMAL FIBROBLASTS; SKIN; LATTICES; MORPHOGENESIS; PROLIFERATION; CONTRACTION; INVITRO; SPONGE; GEL;
Keywords:
liquid crystalline order; collagen fibrils; 3D cell culture; dermal fibroblasts;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Besseau, L Univ Paris 06, Observ Oceanol, F-66650 Banyuls sur Mer, France Univ Paris 06 Banyuls sur Mer France F-66650 sur Mer, France
Citazione:
L. Besseau et al., "Production of ordered collagen matrices for three-dimensional cell culture", BIOMATERIAL, 23(1), 2002, pp. 27-36

Abstract

The aim of this study was to produce collagen gels with controlled fibrillar order as matrices for cell culture. Their structural characterization and colonization by human dermal fibroblasts are presently reported. Ordered matrices are obtained by using the property of type I collagen monomers to self-assemble in liquid crystalline arrays by slow evaporation of acidic solutions at high concentrations. Induction of fibrillogenesis concomittent with the stabilization of the supramolecular order is then obtained, within petri dishes, by gelation of the viscous preparations under ammoniac vapours. For comparison, dermal equivalents, in which collagen compaction dependson fibroblasts contraction, are made according to the method of Bell et al. (Proc. Natl. Acad. Sci. 76(3) (1979) 1274). The fibrillar arrangement of the collagen network in the samples is determined by polarizing optical microscopy and by transmission electron microscopy. Whereas dermal equivalentsexhibit heterogeneous distributions of fibrils, two differents types of order are obtained in the stabilized liquid crystalline collagen samples, namely aligned, i.e. nematic, at 20 mg/ml, or crimped, i.e. precholesteric, at40 mg/ml. The morphology and behaviour of fibroblasts seeded on the surface of the matrices are analysed from day 1 to day 21. The cells are viable, proliferate at the surface of ordered matrices and migrate up to 400 mum indepth. Production of concentrated and ordered collagen matrices provides new perspectives to study the behaviour of cells in a valorized three-dimensional context where the fibrillar organization becomes close to in vivo situations. (C) 2001 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/10/20 alle ore 18:39:49