Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Endothelial cell formation of focal adhesions on hydrophilic plasma polymers
Autore:
Sanborn, SL; Murugesan, G; Marchant, RE; Kottke-Marchant, K;
Indirizzi:
Cleveland Clin Fdn, Dept Clin Pathol, Cleveland, OH 44195 USA Cleveland Clin Fdn Cleveland OH USA 44195 Pathol, Cleveland, OH 44195 USA Case Western Reserve Univ, Dept Biomed Engn, Cleveland, OH 44106 USA Case Western Reserve Univ Cleveland OH USA 44106 Cleveland, OH 44106 USA
Titolo Testata:
BIOMATERIALS
fascicolo: 1, volume: 23, anno: 2002,
pagine: 1 - 8
SICI:
0142-9612(200201)23:1<1:ECFOFA>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
TYROSINE PHOSPHORYLATION; SURFACE WETTABILITY; INITIAL ATTACHMENT; VASCULAR GRAFTS; FIBRONECTIN; KINASE; GROWTH; BIOCOMPATIBILITY; PROLIFERATION; CONFORMATION;
Keywords:
endothelial cells; focal adhesions; paxillin; plasma polymers; stress fibers; vinculin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Kottke-Marchant, K Cleveland Clin Fdn, Dept Clin Pathol, 9500 Euclid Ave, Cleveland, OH 44195USA Cleveland Clin Fdn 9500 Euclid Ave Cleveland OH USA 44195
Citazione:
S.L. Sanborn et al., "Endothelial cell formation of focal adhesions on hydrophilic plasma polymers", BIOMATERIAL, 23(1), 2002, pp. 1-8

Abstract

Endothelial cell (EC) formation and distribution of both actin stress fibers and focal contacts on hydrophilic plasma polymers derived from gamma -butyrolactone (GBL) and n-vinylpyrrolidone (NVP) were examined to determine their ability to support endothelial cell growth in comparison to fibronectin. One hour after seeding, cells adhered and spread moderately on fibronectin with the development of defined actin stress fibers and focal adhesions compared to NVP and GBL, on which the cells were spread with poorly developed stress fibers and a perinuclear localization of vinculin. At 3 h, cells continue to spread more on fibronectin and NVP than GBL, and the cells on fibronectin had well-defined stress fibers terminating with sharp spikes of vinculin, typical of focal adhesions. At this time point, paxillin, a signaling component of focal adhesion complex, was predominantly localized at the focal contacts for well-spread EC on fibronectin and NVP, whereas it was almost entirely concentrated in the perinuclear region of less-spread cellson GBL. However, by 24h, cells were much more spread on all three surfaceswith defined stress fibers and focal contacts although EC expression of vinculin and paxillin was moderate on GBL compared to fibronectin and NVP. These results suggest that EC can form cytoskeletal structures necessary for cell survival on plasma polymers, especially on more hydrophilic NVP, whichcould be exploited as interface material for seeding endothelial cells. (C) 2001 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/04/20 alle ore 20:30:29