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Titolo:
Galactosylated alginate as a scaffold for hepatocytes entrapment
Autore:
Yang, J; Goto, M; Ise, H; Cho, CS; Akaike, T;
Indirizzi:
Tokyo Inst Technol, Dept Biomol Engn, Midori Ku, Yokohama, Kanagawa 2268501, Japan Tokyo Inst Technol Yokohama Kanagawa Japan 2268501 anagawa 2268501, Japan Bio Quest Res Co Ltd, Tokyo 1500043, Japan Bio Quest Res Co Ltd Tokyo Japan 1500043 es Co Ltd, Tokyo 1500043, Japan Shinshu Univ, Grad Sch med, Inst Organ Transplants Reconstruct Med & Tissue E, Matsumoto, Nagano 3908621, Japan Shinshu Univ Matsumoto Nagano Japan 3908621 sumoto, Nagano 3908621, Japan Seoul Natl Univ, Sch Agr Biotechnol, Suwon 441744, South Korea Seoul Natl Univ Suwon South Korea 441744 hnol, Suwon 441744, South Korea
Titolo Testata:
BIOMATERIALS
fascicolo: 2, volume: 23, anno: 2002,
pagine: 471 - 479
SICI:
0142-9612(200201)23:2<471:GAAASF>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
LACTOSE-CARRYING POLYSTYRENE; ADULT-RAT HEPATOCYTES; EXTRACELLULAR-MATRIX; BIOARTIFICIAL LIVER; ADHESION MOLECULE; DRUG CARRIER; CULTURE; CELLS; SPHEROIDS; DIFFERENTIATION;
Keywords:
alginate; synthetic scaffold; galactose; hepatocytes; bioartificial liver; capsules;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Akaike, T Tokyo Inst Technol, Dept Biomol Engn, Midori Ku, 4259 Nagatsuta Cho, Yokohama, Kanagawa 2268501, Japan Tokyo Inst Technol 4259 Nagatsuta Cho Yokohama Kanagawa Japan 2268501
Citazione:
J. Yang et al., "Galactosylated alginate as a scaffold for hepatocytes entrapment", BIOMATERIAL, 23(2), 2002, pp. 471-479

Abstract

Galactose moieties were covalently coupled with alginate through ethylenediamine as the spacer for enhancing the interaction of hepatocytes with alginate, Adhesion of hepatocytes onto the galactosylated alginate (GA)-coated polystyrene (PS) surface showed an 18-fold increase as compared with that of the alginate-coated surface and it increased with an increase in the concentration of GA. The morphologies of attached hepatocytes were observed to spread out at the 0.15 wt% GA-coated PS surface while round cells were observed at the 0.5 wt% GA-coated PS surface. Inhibition of hepatocytes attachment onto the galactose-carrying PS-coated surface occurred with the addition of the GA into the hepatocyte suspension, indicating the binding of GA with hepatocytes via the patch of asialoglycoprotein receptors. Primary hepatocytes were entrapped in the GA/Ca2+ capsules (GAC). Higher cell viability and more spheroid formation of hepatocytes were obtained in the GAC than inthe alginate/Ca2+ capsules (AC). Moreover, liver functions of the hepatocytes such as albumin secretion and urea synthesis in the GAC were improved in comparison with those in the AC. (C) 2001 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/03/20 alle ore 15:26:54