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Titolo:
Organization of cyclic AMP-dependent connexin 43 in Swiss 3T3 cells attached to a cellulose substratum
Autore:
Faucheux, N; Dufresne, M; Nagel, MD;
Indirizzi:
Univ Technol Compiegne, Domaine Biomat Biocompatibil, UMR CNRS 6600, F-60205 Compiegne, France Univ Technol Compiegne Compiegne France F-60205 -60205 Compiegne, France
Titolo Testata:
BIOMATERIALS
fascicolo: 2, volume: 23, anno: 2002,
pagine: 413 - 421
SICI:
0142-9612(200201)23:2<413:OOCAC4>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
GAP-JUNCTIONS; MEMBRANE CHANNEL; PROTEIN-SYNTHESIS; EXPRESSION; COMMUNICATION; PHOSPHORYLATION; GROWTH; CAMP; PERMEABILITY; TRAFFICKING;
Keywords:
gap junction; connexin; cyclic AMP; cell aggregation; cuprophan;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Nagel, MD Univ Technol Compiegne, Domaine Biomat Biocompatibil, UMR CNRS 6600, BP 20529, F-60205 Compiegne, France Univ Technol Compiegne BP 20529 Compiegne France F-60205 France
Citazione:
N. Faucheux et al., "Organization of cyclic AMP-dependent connexin 43 in Swiss 3T3 cells attached to a cellulose substratum", BIOMATERIAL, 23(2), 2002, pp. 413-421

Abstract

We have previously shown that the adenylyl cyclase, which produces cyclic AMP (cAMP) in Swiss 3T3 cells, is activated by their attachment to a cellulose substratum (Cuprophan, CU). This substratum adsorbs vitronectin poorly,prevents cell spreading and causes them to aggregate. By contrast, cells spread out on polystyrene and contain low concentrations of cAMP. We have found that Connexin 43 (Cx 43) gap junction plaques are involved in this cellaggregation. MDL 12330 A, a specific inhibitor of adenylyl cyclase, prevented cell aggregation on CU and abolished Cx 43 channel clustering. But forskolin, a direct activator of adenylyl cyclase, and 8Br cAMP, a cell-permeable analogue of cAMP, caused Cx 43 channel clustering in cells attached to polystyrene. Hence, Cx 43 channel clustering is regulated by cAMP in Swiss 3T3 cells. In addition, neither brefeldin A nor monensin (inhibitors of transit through the endoplasmic reticulum and Golgi apparatus). abolished Cx 43channel clustering in cells aggregated on CU. Thus, the Cx 43 that form clusters in cells attached to CU are not dependent upon the trafficking of Cx43 from intracellular storage sites, but are probably reorganised from theplasma membrane. (C) 2001 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/09/20 alle ore 02:49:41