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Titolo:
Three-dimensional reconstruction of confocal laser microscopy images to study the behaviour of osteoblastic cells grown on biomaterials
Autore:
Ramires, PA; Giuffrida, A; Milella, E;
Indirizzi:
CNRSM, PASTIS, Biomat Unit, I-72100 Brindisi, Italy CNRSM Brindisi ItalyI-72100 ASTIS, Biomat Unit, I-72100 Brindisi, Italy
Titolo Testata:
BIOMATERIALS
fascicolo: 2, volume: 23, anno: 2002,
pagine: 397 - 406
SICI:
0142-9612(200201)23:2<397:TROCLM>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
SURFACE-ROUGHNESS; EXTRACELLULAR-MATRIX; BIOACTIVE GLASS; TITANIUM-ALLOY; INVITRO; FIBROBLASTS; MORPHOLOGY; ATTACHMENT; MINERALIZATION; HYDROXYAPATITE;
Keywords:
3-D image reconstruction; confocal laser scanning microscopy; osteoblastic cells; titanium; titania/hydroxyapatite coating;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Ramires, PA CNRSM, PASTIS, Biomat Unit, SS 7 Appia,Km 714, I-72100 Brindisi, Italy CNRSM SS 7 Appia,Km 714 Brindisi Italy I-72100 rindisi, Italy
Citazione:
P.A. Ramires et al., "Three-dimensional reconstruction of confocal laser microscopy images to study the behaviour of osteoblastic cells grown on biomaterials", BIOMATERIAL, 23(2), 2002, pp. 397-406

Abstract

The adhesion, spreading and cytoskeletal organization of osteoblastic cells seeded onto titanium and titania/hydroxyapatite composite coating (TiO2/HA) were studied using images acquired by confocal laser scanning microscopy. The fluorescence staining technique was employed to visualize actin cytoskeletal organization of cells. 2-D images were exhaustive when the cells were seeded at low density (in the first 24 h of incubation), but they were less clear when the cells proliferated and appeared stacked. Since the shareware software were not satisfactory, a new 3-D image reconstruction was developed using ordinary software and a model was obtained directly from the optical section set, in order to achieve a more realistic and faithful vision of morphological structures and to evaluate the behaviour of bone cells grown on materials. The results showed that the cells grown on titanium conform to the irregular substrate surfaces maximizing the contact between the cell membrane and the substrate and proliferate disposing close to each other. On the contrary, the osteoblasts seeded onto TiO2/HA coating develop clusters where the cells aggregated extending processes in order to establishintercellular connections. Cell aggregation is an early and critical eventleading to cell differentiation and mineralization process and could be a first signal of the tendency of Ti02/11A coating to stimulate cell differentiation, (C) 2001 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/01/20 alle ore 09:46:36