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Titolo:
Polychlorinated biphenyls activate caspase-3-like death protease in vitro but not in vivo
Autore:
Lee, SH; Youk, ES; Jeon, YJ; Han, SB; Kim, HC; Kim, HM;
Indirizzi:
KRIBB, Biopotency Evaluat Lab, Taejon 305333, South Korea KRIBB Taejon South Korea 305333 Evaluat Lab, Taejon 305333, South Korea
Titolo Testata:
BIOLOGICAL & PHARMACEUTICAL BULLETIN
fascicolo: 12, volume: 24, anno: 2001,
pagine: 1380 - 1383
SICI:
0918-6158(200112)24:12<1380:PBACDP>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
MURINE SPLENOCYTES; CELL-LINES; APOPTOSIS; CASPASES; INHIBITION;
Keywords:
polychlorinated biphenyl; caspase-3-like protease; serum albumin; splenocyte;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
21
Recensione:
Indirizzi per estratti:
Indirizzo: Kim, HM KRIBB, Biopotency Evaluat Lab, Taejon 305333, South Korea KRIBB Taejon South Korea 305333 Lab, Taejon 305333, South Korea
Citazione:
S.H. Lee et al., "Polychlorinated biphenyls activate caspase-3-like death protease in vitro but not in vivo", BIOL PHAR B, 24(12), 2001, pp. 1380-1383

Abstract

We prove here that serum albumin inhibits apoptosis induced by polychlorinated biphenyls (PCBs), confirming that serum albumin binds to PCB, and thatthe albumin-PCB complexes inhibit apoptosis in HL-60 cells. We found that PCB (50 muM) increased the activity of caspase-3-like protease when HL-60 cells, as well as splenocytes, were cultured in "serum-free medium. " Benzyloxycarbonyl-Val-Ala-Asp-fluoromethylketone (Z-VAD-fmk) inhibited apoptosis in cells cultured in the serum-free medium containing 50 muM PCB. To elucidate whether or not PCBs induce apoptosis in vivo, we examined apoptosis of splenocytes by administering PCB to ICR mice (100, 500, 1000 mg.kg(-1).d(-1)) for 5d and characterizing splenocytes. Interestingly, splenocytes treatedwith PCB did not show any changes characteristic of apoptosis. These results demonstrate that PCB activates the caspase-3-like death protease in vitro in serum-free medium, but does not induce apoptosis of splenocytes in vivo, suggesting that blood serum may mask the apoptosis induced by PCB.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/09/20 alle ore 13:12:47