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Titolo:
Stearylated arginine-rich peptides: A new class of transfection systems
Autore:
Futaki, S; Ohashi, W; Suzuki, T; Niwa, M; Tanaka, S; Ueda, K; Harashima, H; Sugiura, Y;
Indirizzi:
Kyoto Univ, Inst Chem Res, Kyoto 6110011, Japan Kyoto Univ Kyoto Japan 6110011 Univ, Inst Chem Res, Kyoto 6110011, Japan Hokkaido Univ, Grad Sch Pharmaceut Sci, Sapporo, Hokkaido 0600812, Japan Hokkaido Univ Sapporo Hokkaido Japan 0600812 oro, Hokkaido 0600812, Japan
Titolo Testata:
BIOCONJUGATE CHEMISTRY
fascicolo: 6, volume: 12, anno: 2001,
pagine: 1005 - 1011
SICI:
1043-1802(200111/12)12:6<1005:SAPANC>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
VIVO PROTEIN TRANSDUCTION; ALPHA-HELICAL PEPTIDES; GENE DELIVERY; IN-VIVO; ANTENNAPEDIA HOMEODOMAIN; INTRACELLULAR DELIVERY; PLASMID DNA; 3RD HELIX; CELLS; EXPRESSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Sugiura, Y Kyoto Univ, Inst Chem Res, Kyoto 6110011, Japan Kyoto Univ Kyoto Japan 6110011 Chem Res, Kyoto 6110011, Japan
Citazione:
S. Futaki et al., "Stearylated arginine-rich peptides: A new class of transfection systems", BIOCONJ CHE, 12(6), 2001, pp. 1005-1011

Abstract

Membrane-permeable arginine-rich peptides, such as HIV-1 Tat-(48-60), HIV-1 Rev-(34-50), and flock house virus (FHV) coat-(35-49), have been shown topossess the ability to transfect COS-7 cells with luciferase-coding plasmid as efficiently as polyarginine (MW 5000-15000) and polylysine (MW 9800). Not only these virus-derived cationic peptides but also oligoarginines of 4-16 residues were found to be able to transfect cells. In the case of the Tat, FHV, and octaarginine peptides, N-terminal stearylation of the peptidesincreases the transfection efficiency by approximately 100 times to reach the same order of magnitude as that of LipofectAMINE, one of the most efficient commercially available transfection agents. Also, a certain correlation was observed between the transfection efficiency of stearyl-(Arg)(n) peptides (stearyl-R-n: n = 4, 8, 12, 16) and the membrane permeability of the corresponding (Arg)(n) peptides (R-n).

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/01/20 alle ore 22:07:42