Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Endogenous melanin-concentrating hormone receptor SLC-1 in human melanoma SK-MEL-37 cells
Autore:
Saito, Y; Wang, ZW; Hagino-Yamagishi, K; Civelli, O; Kawashima, S; Maruyama, K;
Indirizzi:
Saitama Med Sch, Dept Pharmacol, Moroyama, Saitama 3500495, Japan Saitama Med Sch Moroyama Saitama Japan 3500495 ma, Saitama 3500495, Japan Univ Calif Irvine, Dept Pharmacol, Irvine, CA 92621 USA Univ Calif IrvineIrvine CA USA 92621 ept Pharmacol, Irvine, CA 92621 USA Tokyo Metropolitan Inst Med Sci, Dept Mol Biol, Bunkyo Ku, Tokyo 1138613, Japan Tokyo Metropolitan Inst Med Sci Tokyo Japan 1138613 Tokyo 1138613, Japan
Titolo Testata:
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
fascicolo: 1, volume: 289, anno: 2001,
pagine: 44 - 50
SICI:
0006-291X(20011123)289:1<44:EMHRSI>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN-COUPLED RECEPTOR; RAT; IDENTIFICATION; LIGAND; MCH; BINDING; SYSTEM; LINE;
Keywords:
G protein-coupled receptor; melanin-concentrating hormone; cyclic AMP; calcium influx; mitogen-activated protein kinase;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Saito, Y Saitama Med Sch, Dept Pharmacol, Moroyama, Saitama 3500495, JapanSaitama Med Sch Moroyama Saitama Japan 3500495 ma 3500495, Japan
Citazione:
Y. Saito et al., "Endogenous melanin-concentrating hormone receptor SLC-1 in human melanoma SK-MEL-37 cells", BIOC BIOP R, 289(1), 2001, pp. 44-50

Abstract

Melanin-concentrating hormone (MCH) is a hypothalamic neuropeptide that regulates several physiological functions. The orphan G protein-coupled receptors SLC-1 and MCHR2 were recently found to bind MCH with high affinity. Weshow here that the human melanoma cell line SK-MEL-37 expresses SLC-1 mRNAbut not MCHR2 by RT-PCR analysis and immunofluorescence studies. Using Chinese hamster ovary cells and 293 cells overexpressing SLC-1 by cDNA transfection, it was shown that SLC-1 coupled to both G alpha (i)/G alpha (o) and G alpha (q) proteins. In SK-MEL-37 cells, MCH inhibited forskolin-stimulated cyclic AMP accumulation and induced mitogen-activated protein kinase (MAPK) in a pertussis toxin- (PTX) -sensitive manner. The MAPK activity leads to the production of phosphorylated forms of p42/p44 MAPK. However, an increase in the intracellular free Ca2+ concentration was not elicited by MCH inSK-MEL-37 cells. These results show that SLC-1 is coupled only to PTX-sensitive Gai/Gao in SK-MEL-37 cells. This study provides for the first time a skin-derived cellular model to analyze the molecular mechanism of the MCH signaling pathway. (C) 2001 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/09/20 alle ore 16:52:13