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Titolo:
Developmental variation in cytochrome P450 expression in Papilio polyxenesin response to xanthotoxin, a hostplant allelochemical
Autore:
Harrison, TL; Zangerl, AR; Schuler, MA; Berenbaum, MR;
Indirizzi:
Univ Illinois, Dept Entomol, Urbana, IL 61801 USA Univ Illinois Urbana ILUSA 61801 ois, Dept Entomol, Urbana, IL 61801 USA Univ Illinois, Dept Cell & Struct Biol, Edward R Madigan Lab 161, Urbana, IL 61801 USA Univ Illinois Urbana IL USA 61801 R Madigan Lab 161, Urbana, IL 61801 USA
Titolo Testata:
ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY
fascicolo: 4, volume: 48, anno: 2001,
pagine: 179 - 189
SICI:
0739-4462(200112)48:4<179:DVICPE>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
PYRETHROID INDUCIBLE CYTOCHROME-P450; MIXED-FUNCTION OXIDASES; HORNWORM MANDUCA-SEXTA; BLACK SWALLOWTAIL; ANGULAR FURANOCOUMARINS; PHYTOPHAGOUS INSECTS; HELICOVERPA-ARMIGERA; FALL ARMYWORM; INDUCTION; DETOXIFICATION;
Keywords:
cytochrome P450; furanocoumarins; Papilio polyxenes; transcriptional regulation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Berenbaum, MR Univ Illinois, Dept Entomol, 320 Morrill Hall,505 S Goodwin,Urbana, IL 61801 USA Univ Illinois 320 Morrill Hall,505 S Goodwin Urbana IL USA 61801
Citazione:
T.L. Harrison et al., "Developmental variation in cytochrome P450 expression in Papilio polyxenesin response to xanthotoxin, a hostplant allelochemical", ARCH INS B, 48(4), 2001, pp. 179-189

Abstract

Although developmental variation in activity and inducibility is typical of cytochrome P450 monooxygenases (P450s) in insects, the adaptive significance of such variation is often unclear, in part because the natural function of insect P450s is rarely known. In this study, we examined developmentalvariation in expression of CYP6B1 and CYP6B3 in Papilio polyxenes, the black swallowtail. Enzymes encoded by these genes have been implicated in the metabolism of xanthotoxin, a furanocoumarin characteristic of the apiaceoushostplants of P. polyxenes. In each life stage-egg, five larval instars, pupa, and adult-we examined individuals exposed to foliage with and without supplemental xanthotoxin. For each stage, we conducted enzyme assays to estimate xanthotoxin metabolism, Northern analysis to detect constitutive and induced mRNA levels, and RT-PCR amplification and Southern analysis to differentiate among P450 genes expressed. Inducible xanthotoxin metabolism, previously reported in fifth instars, was observed in four of five larval stages but was absent or undetectable in all stages that do not feed on foliage; the highest levels of activity were in early larval instars. The same pattern was observed in both Northern and RT-PCR gel blot analyses. In inducible larval stages, inducibility of CYP6B1 transcripts by xanthotoxin was greater than the inducibility of CYP6B3 transcripts. These findings support earlier suggestions that these two P450s contribute to xanthotoxin metabolismin this species and that expression of these P450 genes is regulated in anadaptive fashion with respect to probability of exposure to hostplant toxins over the course of development. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 07:11:41