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Titolo:
Interaction of ammonium, glucose, and chitin regulates the expression of cell wall-degrading enzymes in Trichoderma atroviride strain P1
Autore:
Donzelli, BGG; Harman, GE;
Indirizzi:
Cornell Univ, Dept Hort Sci, Geneva, NY 14456 USA Cornell Univ Geneva NY USA 14456 niv, Dept Hort Sci, Geneva, NY 14456 USA
Titolo Testata:
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
fascicolo: 12, volume: 67, anno: 2001,
pagine: 5643 - 5647
SICI:
0099-2240(200112)67:12<5643:IOAGAC>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
ENDOCHITINASE-ENCODING GENE; BETA-D-GLUCOSAMINIDASE; MYCOPARASITIC INTERACTION; HARZIANUM; BETA-1,3-GLUCANASE; CHITOBIOSIDASE; CLONING; CDNA; CRE1;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
26
Recensione:
Indirizzi per estratti:
Indirizzo: Donzelli, BGG Cornell Univ, Dept Hort Sci, Geneva, NY 14456 USA Cornell Univ Geneva NY USA 14456 Sci, Geneva, NY 14456 USA
Citazione:
B.G.G. Donzelli e G.E. Harman, "Interaction of ammonium, glucose, and chitin regulates the expression of cell wall-degrading enzymes in Trichoderma atroviride strain P1", APPL ENVIR, 67(12), 2001, pp. 5643-5647

Abstract

Chitinolytic and glucanolytic fungal cell wall-degrading enzymes have beensuggested to be primary determinants of biocontrol by Trichoderma spp. We examined the effects of ammonium, glucose, chitin, and chito-oligomers on transcription of specific genes and secretion of fungal cell wall-degrading enzymes. The genes ech42, nag1, and gluc78 were examined, as were the enzymes they encode (endochitinase CHIT42, N-acetylhexosaminidase CHIT73, and glucan exo-1,3-beta -glucanase GLUC78, respectively). gluc78 could be inducedby nitrogen starvation alone, while both ech42 and nag] required nitrogen starvation and the presence of chitin for induction. Starvation for both ammonium and glucose resulted in very early expression and secretion of all cell wall-degrading enzymes examined. In the presence of low levels of ammonium (10 mM), both chito-oligomers and chitin triggered CHIT42 and CHIT40 (chitobiosidase) production. CHIT73 secretion occurred in the presence of N-acetylglucosamine and chito-oligomers, while chitin was less effective. The presence of different chito-oligomers resulted in secretion of specific N-acetylhexosaminidases, of which CHIT73 is one. Our results indicate that theexpression and secretion of cell wall-degrading enzymes is nitrogen repressed, that effects of carbon and nitrogen nutrition are interactive, and that especially for chitinolytic enzymes, the inductive effect of chitin is altered by the level of ammonium or glucose in the medium.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 21:14:10