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Titolo:
Characterization of voltage-gated calcium currents in freshly isolated smooth muscle cells from rat tail main artery
Autore:
Petkov, GV; Fusi, F; Saponara, S; Gagov, HS; Sgaragli, GP; Boev, KK;
Indirizzi:
Bulgarian Acad Sci, Inst Biophys, BU-1113 Sofia, Bulgaria Bulgarian Acad Sci Sofia Bulgaria BU-1113 ophys, BU-1113 Sofia, Bulgaria Univ Vermont, Coll Med, Dept Pharmacol, Burlington, VT 05405 USA Univ Vermont Burlington VT USA 05405 Pharmacol, Burlington, VT 05405 USA Univ Siena, Inst Pharmacol Sci, I-53100 Siena, Italy Univ Siena Siena Italy I-53100 Inst Pharmacol Sci, I-53100 Siena, Italy
Titolo Testata:
ACTA PHYSIOLOGICA SCANDINAVICA
fascicolo: 3, volume: 173, anno: 2001,
pagine: 257 - 265
SICI:
0001-6772(200111)173:3<257:COVCCI>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
GUINEA-PIG; PARATHYROID-HORMONE; HINDERED PHENOLS; CA2+ CHANNELS; AORTA; MIBEFRADIL; INHIBITION; ANTAGONIST; SITES;
Keywords:
Ca2+ channel; cinnarizine; patch-clamp; rat tail artery; vascular smooth muscle;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Boev, KK Bulgarian Acad Sci, Inst Biophys, Acad Georgi Bonchev Str,Bl 21, BU-1113 Sofia, Bulgaria Bulgarian Acad Sci Acad Georgi Bonchev Str,Bl 21 Sofia Bulgaria BU-1113
Citazione:
G.V. Petkov et al., "Characterization of voltage-gated calcium currents in freshly isolated smooth muscle cells from rat tail main artery", ACT PHYSL S, 173(3), 2001, pp. 257-265

Abstract

The aim of the present study was to characterize voltage-gated Ca2+ currents in smooth muscle cells freshly isolated from rat tail main artery in thepresence of 5 mmol L-1 external Ca2+. Calcium currents were identified on the basis of their voltage dependencies and sensitivity to nifedipine, Ni2and cinnarizine. In the majority of the cells studied, T- and L-type currents were observed, while the remaining cells showed predominantly L-type currents. In the latter group of cells, holding potential change from -50 to either -70 or -90 mV increased the corresponding inward current amplitude while its voltage activation threshold remained uncharged. The steady state inactivation of L-type Ca2+ channels showed half-maximal inactivation at -38 mV. A Ca2+ dependent inactivation was also evident. Nifedipine (3 mu mol L-1) blocked L-type but not T-type Ca2+ currents. Ni2+ (50 mu mol L-1) as well as cinnarizine (1 mu mol L-1) suppressed the nifedipine-resistant, T-type component of the currents. At higher concentrations, both Ni2+ (0.3-1 mmol L-1) and cinnarizine (10 mu mol L-1) blocked the net inward current. Replacement of Ca2+ with 10 mmol L-1 Ba2+ significantly increased the amplitude of L-type Ca2+ currents. These results demonstrate that smooth muscle cells freshly isolated from rat tail main artery may be divided into two populations, one expressing both L- and T-type and the other only L-type Ca2+ channels. Furthermore, this report shows that in arterial smooth muscle cellscinnarizine potently inhibited T-type currents at low concentrations (1 mumol L-1) but also blocked L-type Ca2+ currents at higher concentrations (10 mu mol L-1).

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 14/11/18 alle ore 03:12:36