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Titolo:
Effect of the composition of culture media on the laccase production of Lentinus edodes strains
Autore:
Morais, H; Ramos, AC; Cserhati, T; Forgacs, E; Darwish, Y; Illes, Z;
Indirizzi:
Hungarian Acad Sci, Chem Res Ctr, Inst Chem, H-1525 Budapest, Hungary Hungarian Acad Sci Budapest Hungary H-1525 hem, H-1525 Budapest, Hungary Inst Nacl Invest Agr, P-2784505 Nova Oeiras, Portugal Inst Nacl Invest Agr Nova Oeiras Portugal P-2784505 ova Oeiras, Portugal Cent European Univ, Budapest, Hungary Cent European Univ Budapest Hungary nt European Univ, Budapest, Hungary
Titolo Testata:
ACTA BIOTECHNOLOGICA
fascicolo: 4, volume: 21, anno: 2001,
pagine: 307 - 320
SICI:
0138-4988(2001)21:4<307:EOTCOC>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
PRINCIPAL COMPONENT ANALYSIS; BIOLOGICAL-ACTIVITY; DEGRADATION; ENZYMES; LIPOPHILICITY; SURFACTANTS; OXIDATION; MECHANISM; FUNGI; QSAR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Cserhati, T Hungarian Acad Sci, Chem Res Ctr, Inst Chem, POB 17, H-1525 Budapest, Hungary Hungarian Acad Sci POB 17 Budapest Hungary H-1525 st, Hungary
Citazione:
H. Morais et al., "Effect of the composition of culture media on the laccase production of Lentinus edodes strains", ACT BIOTECH, 21(4), 2001, pp. 307-320

Abstract

The effect of charge, ion radii and concentration of cations and fermentation time on the laccase production of four Lentinus edodes strains was determined using 28 different fermentation media and 60 days of fermentation time. Samples were taken every 10 days and the laccase activity was determined by visible spectrophotometry. Principal component analysis (PCA) was usedfor the assessment of similarities and dissimilarities between the laccaseactivities of the samples. As PCA is not suitable for the separation of the strength (potency) and selectivity of the effect of various factors (composition of cultures, fermentation time, Lentinus edodes strains) on the laccase production, they were separated by the spectral mapping technique (SPM). The dimensionality of the matrices of PC loadings and variables and the selectivity maps were reduced to two by the lion-linear mapping technique. The results of PCA and SPM were compared by calculating linear relationships among the potency values and the corresponding coordinates of PCA and SPMmaps. It was established that neither the type of the cations nor their concentration in the fermentation media had a significant effect on the laccase production. It was found that the type of the Lentinus edodes strains and the fermentation time exerts a considerable effect both on the strength and on the selectivity of the laccase production. It was further proven thatthe results of PCA and SPM were considerably different. Therefore, their simultaneous application in future quantitative structure activity relationship (QSAR) studies is highly recommended.

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Documento generato il 01/04/20 alle ore 02:22:59