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Titolo:
Random mutagenesis and use of 2-deoxy-D-glucose as an antimetabolite for selection of alpha-amylase-overproducing mutants of Aspergillus oryzae
Autore:
Azin, M; Noroozi, E;
Indirizzi:
Iranian Res Org Sci & Technol, Ctr Biotechnol, Tehran 15819, Iran Iranian Res Org Sci & Technol Tehran Iran 15819 hnol, Tehran 15819, Iran
Titolo Testata:
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
fascicolo: 7, volume: 17, anno: 2001,
pagine: 747 - 750
SICI:
0959-3993(200110)17:7<747:RMAUO2>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Keywords:
alpha-amylase; Aspergillus oryzae; 2-deoxy-D-glucose; N-methyl-N '-nitro-N-nitrosoguanidine; nitrous acid; random mutagenesis; u.v. light;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
19
Recensione:
Indirizzi per estratti:
Indirizzo: Azin, M Iranian Res Org Sci & Technol, Ctr Biotechnol, POB 15815-3538, Tehran 15819, Iran Iranian Res Org Sci & Technol POB 15815-3538 Tehran Iran 15819 an
Citazione:
M. Azin e E. Noroozi, "Random mutagenesis and use of 2-deoxy-D-glucose as an antimetabolite for selection of alpha-amylase-overproducing mutants of Aspergillus oryzae", WORLD J MIC, 17(7), 2001, pp. 747-750

Abstract

By using 2-deoxy-D-glucose, selection of different mutants of Aspergillus oryzae PTCC 5164, which were produced by random mutagenesis by u.v. radiation, nitrous acid and N-methyl-N'-nitro-N-nitrosoguanidine (MNNG), was studied. 2-Deoxy-D-glucose, a well-known antimetabolite, was used to isolate derepressed mutants. The mutational and lethal effects of these mutagens on conidia of A. oryzae were compared and the frequency distribution of isolatedmutants, in the presence of 2-deoxy-D-glucose, was determined. Potent mutants, which produced higher dextrinizing and saccharogenic activities, were isolated. The best strain was a result of mutagenesis by nitrous acid, which produced 6.73 times more dextrinizing and 5.13 times more saccharogenic activity than the parent strain. In general, the mutants obtained by nitrousacid and u.v. were more potent than those obtained by MNNG.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 15:56:27