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Titolo:
The contribution of molecular biology to the development of vaccines against nematode and trematode parasites of domestic ruminants
Autore:
Knox, DP; Redmond, DL; Skuce, PJ; Newlands, GFJ;
Indirizzi:
Moredun Res Inst, Penicuik EH26 0PZ, Midlothian, Scotland Moredun Res Inst Penicuik Midlothian Scotland EH26 0PZ dlothian, Scotland
Titolo Testata:
VETERINARY PARASITOLOGY
fascicolo: 3-4, volume: 101, anno: 2001,
pagine: 311 - 335
SICI:
0304-4017(20011122)101:3-4<311:TCOMBT>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
GLUTATHIONE-S-TRANSFERASE; ADULT HAEMONCHUS-CONTORTUS; NUCLEIC-ACID VACCINATION; HOST-PROTECTIVE ANTIGEN; CATHEPSIN-L PROTEINASES; PROTEASE GENE FAMILY; FASCIOLA-HEPATICA; GASTROINTESTINAL NEMATODE; TRICHOSTRONGYLUS-COLUBRIFORMIS; TAENIA-OVIS;
Keywords:
molecular biology; nematodes; trematodes; vaccines; recombinant proteins; genomics;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
145
Recensione:
Indirizzi per estratti:
Indirizzo: Knox, DP Moredun Res Inst, Pentlands Sci Pk,Bush Loan, Penicuik EH26 0PZ, Midlothian, Scotland Moredun Res Inst Pentlands Sci Pk,Bush Loan Penicuik Midlothian Scotland EH26 0PZ
Citazione:
D.P. Knox et al., "The contribution of molecular biology to the development of vaccines against nematode and trematode parasites of domestic ruminants", VET PARASIT, 101(3-4), 2001, pp. 311-335

Abstract

Rapid developments in molecular biology have had an enormous impact on theprospects for the development of vaccines to control the major nematode and trematode infestations of livestock. Vaccine candidates are purified using conventional protein chemistry techniques but the limitations imposed by the scarcity of parasite material provide an insurmountable barrier for commercial vaccine production by this means. The ability to purify mRNA from different parasite life-cycle stages and to prepare cDNA expression libraries from it has proven central to the identification of immunogenic parasite proteins, Potentially, protective parasite antigens can now be produced in recombinant form in a variety of vectors and this represents a key breakthrough on the road to commercial vaccine production. The contribution of molecular biology to this process is discussed using several examples, particularly in vaccine development against the pathogenic abomasal nematode of sheep and goats, Haemonchus contortus, and the liver fluke of sheep and cattle, Fasciola hepatica. The difficulties of producing recombinant proteins in the correct form, with appropriate post-translational modification and conformation, are discussed as well as emerging means of antigen delivery including DNA vaccination. The opportunities offered by genome and expressed sequence tag analyses programmes for antigen targeting are discussed in association with developing microarray and proteomics technologies which offer the prospect of large scale, rapid antigen screening and identification. Crown Copyright (C) 2001 Published by Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/04/20 alle ore 13:32:36