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Titolo:
Thiosulfinates inhibit platelet aggregation and microparticle shedding at a calpain-dependent step
Autore:
Rendu, F; Brohard-Bohn, B; Pain, S; Bachelot-Loza, C; Auger, J;
Indirizzi:
Univ Paris 05, U428 INSERM, Fac Pharm, F-75270 Paris 06, France Univ Paris05 Paris France 06 NSERM, Fac Pharm, F-75270 Paris 06, France Univ Tours, IRBI, Tours, France Univ Tours Tours FranceUniv Tours, IRBI, Tours, France
Titolo Testata:
THROMBOSIS AND HAEMOSTASIS
fascicolo: 5, volume: 86, anno: 2001,
pagine: 1284 - 1291
SICI:
0340-6245(200111)86:5<1284:TIPAAM>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN BLOOD-PLATELETS; GLYCOPROTEIN-IIB-IIIA; ONION ALLIUM-CEPA; PLASMA-MEMBRANE; AMINOPHOSPHOLIPID EXPOSURE; MU-CALPAIN; ACTIVATION; INTEGRIN; AJOENE; TALIN;
Keywords:
allium; calpain; microparticles; platelet aggregation; talin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Rendu, F Univ Paris 05, U428 INSERM, Fac Pharm, 4 Ave Observ, F-75270 Paris 06, France Univ Paris 05 4 Ave Observ Paris France 06 5270 Paris 06, France
Citazione:
F. Rendu et al., "Thiosulfinates inhibit platelet aggregation and microparticle shedding at a calpain-dependent step", THROMB HAEM, 86(5), 2001, pp. 1284-1291

Abstract

Thiosulfinates (TSs) are sulfur compounds generated through the processingof different Allium species with antiplatelet property. To further define this platelet inhibitory effect we studied diallyl-TS (Al,TS), dipropyl-TS (Pr2TS), and dimethyl-TS (Me2TS) on platelet responses. The three TSs inhibited dose-dependent platelet aggregation, with IC50 values of 15 +/- 2, 19 /- 2, and 9 +/- 1 muM for Al2TS, Pr2TS and Me2TS, respectively. TSs had noeffect on the expression of a platelet procoagulant surface, measured by flow cytometry as the binding of annexin V-FITC. They inhibited the microparticle shedding and clot retraction. Since the microparticle shedding is a calpain-activation dependent step, we assessed calpain activation by analysis of autoproteolysis in shorter active forms and by talin proteolysis in the presence of TSs. Calpain activation was inhibited by TSs independently offibrinogen binding. Thus, TSs represent a new category of platelet inhibitors, acting on calpain-induced events.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/01/20 alle ore 07:26:13