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Titolo:
The effect of hardening temperature on the stability of mRNA for alpha-subunit of translational elongation factor 1 in wheat and barley seedlings
Autore:
Bakaldina, NB; Alekseenko, ZV; Plotnikov, VK;
Indirizzi:
Russian Acad Agr Sci, Lukyanenko Res Inst Agr, Krasnodar 350012, Russia Russian Acad Agr Sci Krasnodar Russia 350012 r, Krasnodar 350012, Russia
Titolo Testata:
RUSSIAN JOURNAL OF PLANT PHYSIOLOGY
fascicolo: 6, volume: 48, anno: 2001,
pagine: 764 - 769
SICI:
1021-4437(200111/12)48:6<764:TEOHTO>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
MESSENGER-RNA STABILITY; GENE-EXPRESSION; POSTTRANSCRIPTIONAL REGULATION; IN-VITRO; EUKARYOTES; PROTEIN; VIVO;
Keywords:
Triticum aestivum; Hordeum vulgare; differential mRNA stability; poly(A)-containing mRNA; mRNA decay; deadenylation; affinity chromatography; sodium salicylate; alpha-subunit of the translational elongation factor 1;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
20
Recensione:
Indirizzi per estratti:
Indirizzo: Bakaldina, NB Russian Acad Agr Sci, Lukyanenko Res Inst Agr, Krasnodar 350012, Russia Russian Acad Agr Sci Krasnodar Russia 350012 50012, Russia
Citazione:
N.B. Bakaldina et al., "The effect of hardening temperature on the stability of mRNA for alpha-subunit of translational elongation factor 1 in wheat and barley seedlings", RUSS J PL P, 48(6), 2001, pp. 764-769

Abstract

The effects of hardening temperature (4 degreesC) on the stability of total poly(A)-containing mRNA and mRNA for the alpha -subunit of translational elongation factor 1 were studied in seedlings of wheat (Triticum aestivum L.) and barley (Hordeum vulgare L.) cultivars differing in their frost tolerance. The in vitro (ommp) system, which adequately characterizes the relative mRNA stability in vivo was used for these measurements. The effects of cooling on mRNA stability were shown to be cultivar-specific. The ratio between dominant and recessive vrn and ppd genes was assumed to determine the cultivar specificity of the mRNA stability. In frost-tolerant winter wheat and winter barley cultivars, cold-induced changes in the mRNA stability wereopposite in their direction. In winter wheat, sodium salicylate affected the stability of mRNAs for the alpha -subunit or translational elongation factor 1 (eEF-1 alpha), Waxy, and phytochrome A in a cultivar-specific manner. Hardening and sodium salicylate similarly affected the stability of mRNA for eEF-1 alpha, which was shown for four cultivars, Thus, the stability ofmRNA in the ommp system can be considered a new molecular characteristic of the genotype and plant physiological state, which helps to study the post-transcriptional mechanisms of cereal crop adaptation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 12/07/20 alle ore 09:22:34