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Titolo:
Improvement of stability of immobilized GL-7-ACA acylase through modification with glutaraldehyde
Autore:
Park, SW; Kim, YI; Chung, KH; Kim, SW;
Indirizzi:
Korea Univ, Dept Chem Engn, Sungbuk Ku, Seoul 136701, South Korea Korea Univ Seoul South Korea 136701 ungbuk Ku, Seoul 136701, South Korea Chong Kun Dang Pharmaceut Corp, Seoul, South Korea Chong Kun Dang Pharmaceut Corp Seoul South Korea rp, Seoul, South Korea
Titolo Testata:
PROCESS BIOCHEMISTRY
fascicolo: 2, volume: 37, anno: 2001,
pagine: 153 - 163
SICI:
1359-5113(200110)37:2<153:IOSOIG>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
RESPONSE-SURFACE METHODOLOGY; ASPERGILLUS-NIGER; CEPHALOSPORIN-C; OPTIMIZATION; ACID;
Keywords:
immobilization; GL-7-ACA acylase; modification of glutaraldehyde; optimization; analysis of variance; response surface methodology;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Kim, SW Korea Univ, Dept Chem Engn, Sungbuk Ku, 1,Anam Dong, Seoul 136701,South Korea Korea Univ 1,Anam Dong Seoul South Korea 136701 6701, South Korea
Citazione:
S.W. Park et al., "Improvement of stability of immobilized GL-7-ACA acylase through modification with glutaraldehyde", PROCESS BIO, 37(2), 2001, pp. 153-163

Abstract

Glutaryl-7-aminocephalosporanic acid (GL-7-ACA) acylase is an enzyme that converts GL-7-ACA to 7-ACA (7-aminocephalosporanic acid), a starting material for semisynthetic cephalosporin antibiotics. Alleviation of the adverse effect of glutaraldehyde is very important for the enhancement of activity and activity recovery of the immobilized GL-7-ACA acylase. Optimal conditions for the optimization of glutaraldehyde were deduced using statistical analysis, Analysis of variance (ANOVA) was introduced for the determination of significant factors that affect the modification of glutaraldehyde. ANOVAresults suggest the most important factors are the interaction between heating temperature and heating time, and the interaction between pH and glutaraldehyde concentration. Both factors are significant for activity and activity recovery at a 1% level of significance. In order to determine the optimal conditions for significant factors tested by ANOVA, response surface methodology (RSM) was employed. Through RSM analysis, it was found that the optimal conditions for pH. heating temperature, heating time and concentrations of glutaraldehyde for the modification of glutaraldehyde were found to be: pH 8.0. 62 C-degrees, 25 min and 2% concentration of glutaraldehyde, respectively. The values of activity and activity recovery at the optimal points were 18.72 U/g-matrix and 36.52%. respectively. These values for activity and activity recovery were increased up to 2.0- and 2.2-fold, respectively, when compared to those obtained using untreated glutaraldehyde. These increased values were obtained by improvement of immobilization techniques through the modification of glutaraldehyde without the modification of enzyme. As a consequence, this method would be considerably useful in the reuse of immobilized GL-7-ACA acylase for the enhancement of productivity of 7-ACA. More superior effects might be obtained if reuse of immobilized GL-7-ACAacylase should be introduced in scale-up for the mass production of 7-ACA. (C) 2001 Elsevier Science Ltd. All rights reserved.

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Documento generato il 30/09/20 alle ore 09:35:09