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Titolo:
Activation of the epsilon isoform of protein kinase C in the mammalian nerve terminal
Autore:
Saitoh, N; Hori, T; Takahashi, T;
Indirizzi:
Univ Tokyo, Grad Sch Med, Dept Neurophysiol, Tokyo 1130033, Japan Univ Tokyo Tokyo Japan 1130033 , Dept Neurophysiol, Tokyo 1130033, Japan
Titolo Testata:
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
fascicolo: 24, volume: 98, anno: 2001,
pagine: 14017 - 14021
SICI:
0027-8424(20011120)98:24<14017:AOTEIO>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
PHORBOL ESTER RECEPTOR; SYNAPTIC TRANSMISSION; TRANSMITTER RELEASE; IN-VITRO; NEUROTRANSMITTER RELEASE; PRESYNAPTIC LOCALIZATION; RAT HIPPOCAMPUS; POTENTIATION; ACTIN; CELLS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
44
Recensione:
Indirizzi per estratti:
Indirizzo: Takahashi, T Univ Tokyo, Grad Sch Med, Dept Neurophysiol, Tokyo 1130033, Japan Univ Tokyo Tokyo Japan 1130033 hysiol, Tokyo 1130033, Japan
Citazione:
N. Saitoh et al., "Activation of the epsilon isoform of protein kinase C in the mammalian nerve terminal", P NAS US, 98(24), 2001, pp. 14017-14021

Abstract

Activation of protein kinase C (PKC) by phorbol ester facilitates hormonalsecretion and transmitter release, and phorbol ester-induced synaptic potentiation (PESP) is a model for presynaptic facilitation. A variety of PKC isoforms are expressed in the central nervous system, but the isoform involved in the PESP has not been identified. To address this question, we have applied immunocytochemical and electrophysiological techniques to the calyx of Held synapse in the nrl nucleus of the trapezoid body (MNTB) of rat auditory brainstem. Western blot analysis indicated that both the Ca2+-dependent "conventional" PKC and Ca2+-independent "novel" PKC isoforms are expressed in the MNTB. Denervation of afferent fibers followed by organotypic culture, however, selectively decreased "novel" epsilon PKC isoform expressed inthis region. The afferent calyx terminal was clearly labeled with the epsilon PKC immunofluorescence. On stimulation with phorbol ester, presynaptic epsilon PKC underwent autophosphorylation and unidirectional translocation toward the synaptic side. Chelating presynaptic Ca2+, by using membrane permeable EGTA analogue or high concentration of EGTA directly loaded into calyceal terminals, had only a minor attenuating effect on the PESP. We conclude that the Ca2+-independente epsilon PKC isoform mediates the PESP at thismammalian central nervous system synapse.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 02:53:17