Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Growing of potato microplants in the presence of alginate-silverthiosulfate capsules reduces ethylene-induced culture abnormalities during minimal growth conservation in vitro
Autore:
Sarkar, D; Sud, KC; Chakrabarti, SK; Naik, PS;
Indirizzi:
Cent Potato Res Inst, Div Crop Improvement, Shimla 171001, Himachal Prades, India Cent Potato Res Inst Shimla Himachal Prades India 171001 al Prades, India
Titolo Testata:
PLANT CELL TISSUE AND ORGAN CULTURE
fascicolo: 1, volume: 68, anno: 2002,
pagine: 79 - 89
SICI:
0167-6857(200201)68:1<79:GOPMIT>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
IN-VITRO; SILVER THIOSULFATE; INVITRO; STORAGE; REGENERATION; GERMPLASM; PLANTS; BIOSYNTHESIS; GENERATION; TISSUE;
Keywords:
alginate capsules; ethylene inhibition; germplasm conservation; in vitro conservation; radioactive silver; silverthiosulfate; Solanum tuberosum L;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Sarkar, D Cent Potato Res Inst, Div Crop Improvement, Shimla 171001, Himachal Prades, India Cent Potato Res Inst Shimla Himachal Prades India 171001 India
Citazione:
D. Sarkar et al., "Growing of potato microplants in the presence of alginate-silverthiosulfate capsules reduces ethylene-induced culture abnormalities during minimal growth conservation in vitro", PL CELL TIS, 68(1), 2002, pp. 79-89

Abstract

Alginate capsules containing anionic complex silverthiosulfate (STS) [Ag(S2O3)(2)(3-)] were placed in the culture tubes over minimal growth media forstudying whether STS could be used at higher concentrations to sustain ethylene-inhibiting effect during conservation of microplants in six potato (Solanum tuberosum L.) genotypes in vitro. Different concentrations of STS (0.1, 0.5, 1.0, 2.0, 3.0 and 4.0 mM) were incorporated into the alginate capsules, and 12 alginate-STS capsules were tested in semisolid (7 g l(-1) agar) minimal growth medium containing 20 g l(-1) mannitol and 40 g l(-1) sucrose. This indirect supplementation of STS through alginate capsules renderedreduced total availability of STS in the minimal growth medium as comparedto when it was directly supplemented in the medium at a given concentration. Growing of microplants in the presence of alginate-STS capsules improvedthe microplant growth and reduced the culture abnormalities over a period of 16 months under minimal growth conditions. Most significant improvement in microplant growth was in terms of green leaf production and leaf senescence. Vitrification, flaccidity and other growth abnormalities, viz., leaf loss, abnormal stem swelling and necrosis were not observed when the microplants were conserved in the presence of alginate-STS capsules. To foster optimum microplant growth and reduce culture abnormalities, potato microplantscould favourably be maintained in the presence of 0.5-1.0 mM alginate-STS capsules during minimal growth conservation. Higher concentrations of alginate-STS capsules (>1.0 mM) were in general detrimental to potato microplantgrowth and survival during prolonged storage in vitro. Release kinetics ofSTS from the alginate-STS capsules, its distribution in the medium and accumulation of silver in potato microplants were studied using (110)mAg. The release rate of STS from the capsules was found to be directly proportionalto the concentrations of alginate-STS capsules. A distinct concentration gradient of (110)mAg in the medium with increasing depth from top to bottom,and its accumulation in the potato microplants may be attributed to the improved anti-ethylene action of STS at higher concentrations through alginate capsules.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/03/20 alle ore 09:27:04