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Titolo:
Functional analysis of salt-inducible proline transporter of barley roots
Autore:
Ueda, A; Shi, WM; Sanmiya, K; Shono, M; Takabe, T;
Indirizzi:
Nagoya Univ, Grad Sch Bioagr Sci, Chikusa Ku, Nagoya, Aichi 4648601, JapanNagoya Univ Nagoya Aichi Japan 4648601 a Ku, Nagoya, Aichi 4648601, Japan Japan Int Res Ctr Agr Sci, Ishigaki 9070002, Japan Japan Int Res Ctr Agr Sci Ishigaki Japan 9070002 Ishigaki 9070002, Japan Nagoya Univ, Biosci Ctr, Chikusa Ku, Nagoya, Aichi 4648601, Japan Nagoya Univ Nagoya Aichi Japan 4648601 a Ku, Nagoya, Aichi 4648601, Japan
Titolo Testata:
PLANT AND CELL PHYSIOLOGY
fascicolo: 11, volume: 42, anno: 2001,
pagine: 1282 - 1289
SICI:
0032-0781(200111)42:11<1282:FAOSPT>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
SALMONELLA-TYPHIMURIUM; ARABIDOPSIS-THALIANA; AMINO-ACID; GENE; BETAINE; PLANTS; STRESS; SYSTEM; OSMOREGULATION; DEHYDROGENASE;
Keywords:
Hordeum vulgare; proline transporter; salt stress; differential display; in situ hybridization;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Takabe, T Nagoya Univ, Grad Sch Bioagr Sci, Chikusa Ku, Nagoya, Aichi 4648601, Japan Nagoya Univ Nagoya Aichi Japan 4648601 ya, Aichi 4648601, Japan
Citazione:
A. Ueda et al., "Functional analysis of salt-inducible proline transporter of barley roots", PLANT CEL P, 42(11), 2001, pp. 1282-1289

Abstract

We cloned a cDNA encoding Hordeum vulgare Proline Transporter (HvProT) from salt-stressed barley roots by differential display. HvProT was 2,161 bp long and had an open reading frame encoding 450 amino acids. The deduced amino acid sequence of HvProT was similar to those of proline transporter proteins of rice (65.7%), Arabidopsis (57.7%) and tomato (42.0%). Northern blotanalysis showed that the transcript level of HvProT was induced in roots at 30 min after 200 mM NaCl treatment and its peak was observed at 3 h. However, the transcript level was very low in leaves and did not increase by salt stress. The expression level of Delta (1)-pyrroline-5-carboxylate synthetase (P5CS), encoding a key enzyme of proline synthesis, was induced later than HvProT by salt stress. A transport assay using a yeast with mutation in proline uptake revealed that HvProT was a transporter with high affinity for (L)-proline (K-m = 25 muM). HvProT was found to be a unique transporterwith high affinity for (L)-proline. Since its transport activity was dependent on the pH gradient, HvProT was suggested to be a H+/amino acid symporter. In situ hybridization analysis showed that the HvProT mRNA was stronglyexpressed in root cap cells under salt stress. HvProT might play an important role in the transport of proline to root tip region urgently upon salt stress.

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Documento generato il 11/07/20 alle ore 16:46:10