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Titolo:
A basic peroxidase from wheat kernel with antifungal activity
Autore:
Caruso, C; Chilosi, G; Leonardi, L; Bertini, L; Magro, P; Buonocore, V; Caporale, C;
Indirizzi:
Univ Tuscia, Dipartimento Agrobiol & Agrochim, I-01100 Viterbo, Italy UnivTuscia Viterbo Italy I-01100 iol & Agrochim, I-01100 Viterbo, Italy Univ Tuscia, Dipartimento Protez Piante, I-01100 Viterbo, Italy Univ Tuscia Viterbo Italy I-01100 Protez Piante, I-01100 Viterbo, Italy
Titolo Testata:
PHYTOCHEMISTRY
fascicolo: 5, volume: 58, anno: 2001,
pagine: 743 - 750
SICI:
0031-9422(200111)58:5<743:ABPFWK>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
TISSUE-SPECIFIC EXPRESSION; HIGHLY ANIONIC PEROXIDASE; TRITICUM-AESTIVUM L; ORYZAE PV ORYZAE; MOLECULAR-CLONING; HORSERADISH-PEROXIDASE; PUTATIVE PEROXIDASE; CYANOGEN-BROMIDE; AMINO-ACID; INDUCTION;
Keywords:
Gramineae; Triticum aestivum; wheat seeds; antifungal activity; amino acid sequence; basic peroxidase;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Caruso, C Univ Tuscia, Dipartimento Agrobiol & Agrochim, Via S Camillo Lellis, I-01100 Viterbo, Italy Univ Tuscia Via S Camillo Lellis Viterbo ItalyI-01100 o, Italy
Citazione:
C. Caruso et al., "A basic peroxidase from wheat kernel with antifungal activity", PHYTOCHEM, 58(5), 2001, pp. 743-750

Abstract

A basic heme-peroxidase (WP1) was purified to homogeneity from wheat (Triticum aestivum) kernels. The protein was not glycosylated and exhibited a molecular mass of 36 kDa and a pl of 8.0. The N-terminal amino acid sequence revealed a very high similarity with a wheat flour peroxidase allergen associated with baker's asthma. WPI showed indole-3-acetic acid oxidase activity in the presence of Mn2+ and phenolic cofactors. Antifungal assays performed in vitro towards phytopathogenic fungi indicated that WPI was active in inhibiting germ tube elongation. This first report on antifungal propertiesof a heme-peroxidase gives experimental support to the idea that peroxidases play a defensive role against invading pathogens. (C) 2001 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/10/20 alle ore 07:38:04