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Titolo:
Autoxidation and toxicant-induced oxidation of lipid and DNA in monkey liver: Reduction of molecular damage by melatonin
Autore:
Cabrera, J; Burkhardt, S; Tan, DX; Manchester, LC; Karbownik, M; Reiter, RJ;
Indirizzi:
Univ Texas, Hlth Sci Ctr, Dept Cellular & Struct Biol, San Antonio, TX 78229 USA Univ Texas San Antonio TX USA 78229 truct Biol, San Antonio, TX 78229 USA Univ Las Palmas Grand Canary, Fac Med, Dept Pharmacol, Las Palmas Gran Canaria 35080, Spain Univ Las Palmas Grand Canary Las Palmas Gran Canaria Spain 35080 , Spain
Titolo Testata:
PHARMACOLOGY & TOXICOLOGY
fascicolo: 5, volume: 89, anno: 2001,
pagine: 225 - 230
SICI:
0901-9928(200111)89:5<225:AATOOL>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
PINEAL HORMONE MELATONIN; FREE-RADICALS; HYDROGEN-PEROXIDE; OXYGEN RADICALS; HEPATIC-INJURY; IN-VIVO; DISEASE; STRESS; RATS; ANTIOXIDANTS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
59
Recensione:
Indirizzi per estratti:
Indirizzo: Reiter, RJ Univ Texas, Hlth Sci Ctr, Dept Cellular & Struct Biol, 7703 Floyd Curl Dr,Mail Code 7762, San Antonio, TX 78229 USA Univ Texas 7703 Floyd Curl Dr,Mail Code 7762 San Antonio TX USA 78229
Citazione:
J. Cabrera et al., "Autoxidation and toxicant-induced oxidation of lipid and DNA in monkey liver: Reduction of molecular damage by melatonin", PHARM TOX, 89(5), 2001, pp. 225-230

Abstract

Melatonin, the main secretory product of the pineal gland, is a free radical scavenger and antioxidant which protects against oxidative damage due toa variety of toxicants. However, there is little information regarding melatonin's antioxidative capacity in tissues of primates. In this study we examined the protective effects of melatonin in monkey liver homogenates against lipid damage that occurred as a result of autoxidation or that induced by exogenous addition of H2O2 and ferrous iron (Fe2+). Additionally, we tested melatonin's protective effect against oxidative damage to DNA induced by chromium(III) (CrIII) plus H2O2. The levels of malondialdehyde and 4-hydroxyalkenals were assayed as an index of lipid peroxidation, and the concentrations of 8-hydroxydeoxyguanosine (8-OHdG) as an endpoint of oxidative DNAdamage. The increases in malondialdehyde+4-hydroxyalkenals concentrations as a consequence of autoxidation or after the addition of H2O2 plus Fe2+ tothe homogenates were time-dependent. The accumulation of these damaged products due to either autoxidative processes or induced by H2O2 and Fe2+ weresignificantly reduced by melatonin in a concentration-dependent-manner. The levels of 8-OHdG were elevated in purified monkey liver DNA incubated with a combination of CrCl3 plus H2O2. This rise in oxidatively damaged DNA was prevented by 10 muM concentration of melatonin. Also, melatonin reduced the damage to DNA that was caused by autoxidative processes. These findings in monkey liver tissue document the ability of melatonin to protect againstoxidative damage to both lipid and DNA in primate tissue, as observed previously in rodent tissue. The findings provide support for the use of melatonin as suitable agent to reduce damage inflicted by free radical species inprimates.

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Documento generato il 14/08/20 alle ore 16:15:32