Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Functional p53 is required for triptolide-induced apoptosis and AP-1 and nuclear factor-kappa B activation in gastric cancer cells
Autore:
Jiang, XH; Wong, BCY; Lin, MCM; Zhu, GH; Kung, HF; Jiang, SH; Yang, D; Lam, SK;
Indirizzi:
Univ Hong Kong, Queen Mary Hosp, Dept Med, Hong Kong, Hong Kong, Peoples RChina Univ Hong Kong Hong Kong Hong Kong Peoples R China Kong, Peoples RChina Rui Jin Hosp, Dept Gastroenterol, Shanghai, Peoples R China Rui Jin Hosp Shanghai Peoples R China nterol, Shanghai, Peoples R China Univ Hong Kong, Inst Mol Biol, Hong Kong, Hong Kong, Peoples R China Univ Hong Kong Hong Kong Hong Kong Peoples R China Kong, Peoples R China Univ Hong Kong, Dept Chem, Hong Kong, Hong Kong, Peoples R China Univ HongKong Hong Kong Hong Kong Peoples R China Kong, Peoples R China
Titolo Testata:
ONCOGENE
fascicolo: 55, volume: 20, anno: 2001,
pagine: 8009 - 8018
SICI:
0950-9232(20011129)20:55<8009:FPIRFT>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
WILD-TYPE P53; NECROSIS-FACTOR-ALPHA; TRIPTERYGIUM-WILFORDII; GAMMA-IRRADIATION; TRANSCRIPTION; INDUCTION; ARREST; GENE; PHOSPHORYLATION; TRANSFORMATION;
Keywords:
apoptosis; NF-kappa B; AP-1; p53 status; gastric cancer;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Wong, BCY Univ Hong Kong, Queen Mary Hosp, Dept Med, Hong Kong, Hong Kong,Peoples RChina Univ Hong Kong Hong Kong Hong Kong Peoples R China ples RChina
Citazione:
X.H. Jiang et al., "Functional p53 is required for triptolide-induced apoptosis and AP-1 and nuclear factor-kappa B activation in gastric cancer cells", ONCOGENE, 20(55), 2001, pp. 8009-8018

Abstract

Triptolide, a major component in the extract of Chinese herbal plant Tripterygium wilfordii Hook f (TWHf), has potential anti-neoplastic effect. In the present study we investigated the potential therapeutic effects and mechanisms of triptolide against human gastric cancer cells. Four gastric cancer cell lines with different p53 status, AGS and MKN-45 (wild type p53); MKN-28 and SGC-7901 (mutant p53) were observed as to cell growth inhibition and induction of apoptosis in response to triptolide treatment. We showed that triptolide inhibited cell growth, induced apoptosis and suppressed NK-kappaB and AP-1 transactivation in AGS cells with wild-type p53. Triptolide induced apoptosis by stimulating the expressions of p53, p21(waf1/cip1), bax protein, and increased the activity of caspases. In addition, it caused cell cycle arrest in the G(0)/G(1) phase. To examine the role of p53 in these functions, we showed that suppression of p53 level with antisense oligonucleotide abrogated triptolide-induced apoptosis and over-expression of dominant negative p53 abolished the inhibitory effect on NF-kappa dB activation. Furthermore, we demonstrated that triptolide had differential effects on gastric cancer cells with different p53 status. We showed that triptolide also inhibited cell growth and induced apoptosis in MKN-45 with wild-type p53,whereas it had no significant growth-inhibition and apoptosis induction effects on the MKN-28 and SGC-7901 cells with mutant p53. Our data suggest that triptolide exhibits anti-tumor and anti-inflammatory effects by inhibiting cell proliferation, inducing apoptosis and inhibiting NF-kappaB and AP-1transcriptional activity. However, a functional p53 is required for these proapoptotic, anti-inflammatory and anti-tumor effects.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/01/20 alle ore 15:47:26