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Titolo:
Presynaptic localization of the carboxy-terminus epitopes of the mu opioidreceptor splice variants MOR-1C and MOR-1D in the superficial laminae of the rat spinal cord
Autore:
Abbadie, C; Pasternak, GW; Aicher, SA;
Indirizzi:
Mem Sloan Kettering Canc Ctr, Dept Neurol, Lab Mol Neuropharmacol, New York, NY 10021 USA Mem Sloan Kettering Canc Ctr New York NY USA 10021 New York, NY 10021 USA Cornell Univ, Weill Med Coll, Dept Neurol & Neurosci, New York, NY 10021 USA Cornell Univ New York NY USA 10021 rol & Neurosci, New York, NY 10021 USA Cornell Univ, Weill Med Coll, Dept Pharmacol & Psychiat, New York, NY 10021 USA Cornell Univ New York NY USA 10021 col & Psychiat, New York, NY 10021 USA
Titolo Testata:
NEUROSCIENCE
fascicolo: 4, volume: 106, anno: 2001,
pagine: 833 - 842
SICI:
0306-4522(2001)106:4<833:PLOTCE>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
STIMULI-INDUCED RELEASE; DORSAL-ROOT GANGLIA; SUBSTANCE-P RELEASE; ULTRASTRUCTURAL-LOCALIZATION; EVOKED RELEASE; AXON TERMINALS; MESSENGER-RNA; MORPHINE; HORN; EXPRESSION;
Keywords:
double labeling; dorsal horn; electron microscopy; immunohistochemistry; mu opioid receptor; splice variant;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Pasternak, GW Mem Sloan Kettering Canc Ctr, Dept Neurol, Lab Mol Neuropharmacol, 1275 York Ave, New York, NY 10021 USA Mem Sloan Kettering Canc Ctr 1275 York Ave New York NY USA 10021
Citazione:
C. Abbadie et al., "Presynaptic localization of the carboxy-terminus epitopes of the mu opioidreceptor splice variants MOR-1C and MOR-1D in the superficial laminae of the rat spinal cord", NEUROSCIENC, 106(4), 2001, pp. 833-842

Abstract

Opioids inhibit nociceptive transmission at the level of the spinal cord, possibly through inhibition of neurotransmitter release by presynaptic mu opioid receptors (MORs) thus preventing the activation of ascending pathwaysand the perception of pain. Most nociceptive primary afferents are unmyelinated fibers containing peptides such as substance P and/or calcitonin gene-related peptide. However, few terminals contain both substance P and MOR. Recently, we identified new carboxy-terminal MOR splice variants that are localized in the superficial laminae of the dorsal horn. We now report the precise cellular distribution of two of these MOR-1 variants, MOR-1C (exon 7/8/9 epitope) and MOR-ID (exon 8/9 epitope), at the ultrastructural level. In the superficial laminae of the dorsal horn, the majority of the labelingof MOR-1C and MOR-1D was found in unmyelinated axons. This distribution contrasts with that of MOR-1 (exon 4 epitope), in which labeling is equally found in dendrites. and soma, as well as in axons. The presence of dense core vesicles in many of the MOR-IC-like immunoreactive terminals implies thatthis splice variant might be involved in presynaptic inhibition of transmitter release from peptide-containing afferents to the dorsal horn. Consistent with this finding, confocal microscopy analyses showed that many MOR-IC profiles in laminae I-II also contained calcitonin gene-related peptide, whereas fewer MOR-1 profiles contained either substance P or calcitonin gene-related peptide in this same region. From these findings we suggest that there are differential distributions of MOR-1 splice variants as well as distinct peptide colocalizations in the dorsal horn. (C) 2001 IBRO. Published by Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 11:11:34