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Titolo:
The sulphydryl reagent, N-ethylmaleimide, disrupts sleep and blocks A1 adenosine receptor-mediated inhibition of intracellular calcium signaling in the in vitro ventromedial preoptic nucleus
Autore:
Ikeda, M; Sagara, M; Sekino, Y; Shirao, T; Honda, K; Yoshioka, T; Allen, CN; Inoue, S;
Indirizzi:
Oregon Hlth Sci Univ, Ctr Res Occupat & Environm Toxicol, Portland, OR 97201 USA Oregon Hlth Sci Univ Portland OR USA 97201 oxicol, Portland, OR 97201 USA Waseda Univ, Adv Res Inst Sci & Engn, Shinjyuku Ku, Tokyo 1698555, Japan Waseda Univ Tokyo Japan 1698555 Engn, Shinjyuku Ku, Tokyo 1698555, Japan Tokyo Med & Dent Univ, Inst Biomat & Bioengn, Tokyo 1010062, Japan Tokyo Med & Dent Univ Tokyo Japan 1010062 Bioengn, Tokyo 1010062, Japan Waseda Univ, Sch Human Sci, Saitama 3591192, Japan Waseda Univ Saitama Japan 3591192 Sch Human Sci, Saitama 3591192, Japan Gunma Univ, Sch Med, Dept Neurobiol & Behav, Maebashi, Gumma 3718511, Japan Gunma Univ Maebashi Gumma Japan 3718511 v, Maebashi, Gumma 3718511, Japan
Titolo Testata:
NEUROSCIENCE
fascicolo: 4, volume: 106, anno: 2001,
pagine: 733 - 743
SICI:
0306-4522(2001)106:4<733:TSRNDS>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROSTAGLANDIN-D SYNTHASE; BASAL FOREBRAIN NEURONS; SENSITIVE G-PROTEINS; UNRESTRAINED RATS; SUPRACHIASMATIC NUCLEUS; SYMPATHETIC NEURONS; REM-SLEEP; BRAIN; AREA; MODULATION;
Keywords:
Ca2+ imaging; fura-2; GTP-binding protein; immunostaining; intracerebroventricular infusion; voltage-sensitive Ca2+ channels;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
60
Recensione:
Indirizzi per estratti:
Indirizzo: Ikeda, M Oregon Hlth Sci Univ, Ctr Res Occupat & Environm Toxicol, 3181 Sam JacksonPk Rd,L606, Portland, OR 97201 USA Oregon Hlth Sci Univ 3181 Sam Jackson Pk Rd,L606 Portland OR USA 97201
Citazione:
M. Ikeda et al., "The sulphydryl reagent, N-ethylmaleimide, disrupts sleep and blocks A1 adenosine receptor-mediated inhibition of intracellular calcium signaling in the in vitro ventromedial preoptic nucleus", NEUROSCIENC, 106(4), 2001, pp. 733-743

Abstract

To explore the neuronal signaling mechanisms underlying sleep regulation in the rat, the present study examined continuous intra-third ventricle infusion of N-ethylmaleimide (NEM), a sulphydryl reagent that inhibits G(i/o) protein-coupled receptor-mediated signaling pathways. The diurnal infusion of NEM (0.01-10 mu mol/10 h) dose-dependently inhibited both non-rapid eye movement sleep and rapid eye movement sleep. A maximal dose of NEM (10 mu mol/10 h) dramatically inhibited day-time sleep (-57% for non-rapid eye movement sleep and -89% for rapid eye movement sleep) with a compensatory increase of sleep during the subsequent night-time (+33% for non-rapid eye movement sleep and +259% for rapid eye movement sleep). The day-time brain temperature was also increased by NEM, demonstrating effects of NEM on both sleepand body temperature levels. Immunostaining of the rat hypothalamus with amonoclonal antibody against the Al adenosine receptor (AIR) was used to explore the distribution of a sleep-related G(i/o) protein-coupled receptor. Robust AIR-like immunoreactivity was found in the ventromedial preoptic nucleus and the supraoptic nucleus. Fura-2-based Ca2+ imaging analysis of acute hypothalamic slices further demonstrated that the AIR agonist N-6-cyclopentyladenosine (CPA; 200 nM) inhibited spontaneous Ca2+ oscillations and high potassium (80 mM)-induced Ca2+ flux in the ventromedial preoptic nucleus,while NEM (100-300 LM) and an AIR antagonist 8-cyclopentyl-dipropylxanthine (300 nM) blocked the CPA actions and increased the high potassium-inducedCa2+ flux. From these results we suggest that NEM-sensitive G protein-coupled receptor(s) may play an important role in the regulation of sleep and body temperature in the rat and one possible mechanism is an AIR-mediated regulation ofintracellular Ca2+ concentrations in the ventromedial preoptic nucleus. (C) 2001 IBRO. Published by Elsevier Science Ltd. All rights reserved.

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Documento generato il 29/03/20 alle ore 15:27:50