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Titolo:
ATP-gated ion channel P2X(3) is increased in human inflammatory bowel disease
Autore:
Yiangou, Y; Facer, P; Baecker, PA; Ford, AP; Knowles, CH; Chan, CLH; Williams, NS; Anand, P;
Indirizzi:
Hammersmith Hosp, Imperial Coll Sch Med, Peripheral Neuropathy Unit, London W12 0NN, England Hammersmith Hosp London England W12 0NN hy Unit, London W12 0NN, England Roche Biosci, Neurobiol Unit, Palo Alto, CA USA Roche Biosci Palo Alto CAUSA Biosci, Neurobiol Unit, Palo Alto, CA USA St Bartholomews & royal london Sch Med & Dent, Acad Dept Surg, London, England St Bartholomews & royal london Sch Med & Dent London England n, England
Titolo Testata:
NEUROGASTROENTEROLOGY AND MOTILITY
fascicolo: 4, volume: 13, anno: 2001,
pagine: 365 - 369
SICI:
1350-1925(200108)13:4<365:AICPII>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
SENSORY NEURONS; URINARY-BLADDER; PURINERGIC RECEPTORS; NEUROTROPHIC FACTOR; NERVOUS-SYSTEM; RAT RETINA; PURINOCEPTORS; LOCALIZATION; EXPRESSION; INTESTINE;
Keywords:
adenosine 5 '-triphosphate; immunohistochemistry; inflammatory bowel disease; P2X(3); Western blotting;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Anand, P Hammersmith Hosp, Imperial Coll Sch Med, Peripheral Neuropathy Unit, Du Cane Rd, London W12 0NN, England Hammersmith Hosp Du Cane Rd LondonEngland W12 0NN 0NN, England
Citazione:
Y. Yiangou et al., "ATP-gated ion channel P2X(3) is increased in human inflammatory bowel disease", NEUROG MOT, 13(4), 2001, pp. 365-369

Abstract

P2X(3) is a novel ATP-gated cation channel that is selectively expressed by small-diameter sensory neurones in rodents, and may play a role in nociception by binding ATP released from damaged or inflamed tissues. We, have studied, for the first time, P2X(3) immunoreactivity in human inflammatory bowel disease, using Western blotting and immunohistochemistry. A major 66-kDa specific protein was found by Western blotting in all colon extracts. In the inflamed group there was a significant two-fold increase in the relative optical density of the 66-kDa band (21.2 +/- 3.1; n = 8) compared to controls (11.4 +/- 3.7, n=8; P=0.009). In the control colon, P2X(3)-immunoreactive neurones were scattered throughout the myenteric and submucosal plexuses, with some neurones showing immunopositive axons/dendrites. The pattern of immunostaining was similar to the neuronal marker peripherin. In general,the intensity of the staining was greater in myenteric than submucosal neurones. The number of P2X(3)-immunoreactive neurones was significantly increased in the myenteric plexus of inflamed colon compared to controls (n = 13; P=0.01). In humans, unlike rodents, P2X(3) is thus not restricted to sensory neurones, Increased P2X(3) in inflamed intestine suggests a potential role in dysmotility and pain, for which it represents a new therapeutic target.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/03/20 alle ore 00:55:07