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Titolo:
Multisite phosphorylation of a CDK inhibitor sets a threshold for the onset of DNA replication
Autore:
Nash, P; Tang, XJ; Orlicky, S; Chen, QH; Gertler, FB; Mendenhall, MD; Sicheri, F; Pawson, T; Tyers, M;
Indirizzi:
Mt Sinai Hosp, Samuel Lunenfeld Res Inst, Programme Mol Biol & Canc, Toronto, ON M5G 1X5, Canada Mt Sinai Hosp Toronto ON Canada M5G 1X5 Canc, Toronto, ON M5G 1X5, Canada Univ Kentucky, Dept Biochem, LP Markey Canc Ctr, Lexington, KY 40536 USA Univ Kentucky Lexington KY USA 40536 ey Canc Ctr, Lexington, KY 40536 USA MIT, Dept Biol, Cambridge, MA 02139 USA MIT Cambridge MA USA 02139MIT, Dept Biol, Cambridge, MA 02139 USA Univ Toronto, Dept Med Genet & Microbiol, Toronto, ON M5S 1A8, Canada UnivToronto Toronto ON Canada M5S 1A8 obiol, Toronto, ON M5S 1A8, Canada
Titolo Testata:
NATURE
fascicolo: 6863, volume: 414, anno: 2001,
pagine: 514 - 521
SICI:
0028-0836(20011129)414:6863<514:MPOACI>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
UBIQUITIN-LIGASE COMPLEX; PROTEIN-PROTEIN INTERACTIONS; CELL-CYCLE ARREST; SACCHAROMYCES-CEREVISIAE; BUDDING YEAST; F-BOX; DEPENDENT DEGRADATION; S-PHASE; KAPPA-B; KINASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Physical, Chemical & Earth Sciences
Citazioni:
48
Recensione:
Indirizzi per estratti:
Indirizzo: Pawson, T Mt Sinai Hosp, Samuel Lunenfeld Res Inst, Programme Mol Biol & Canc, 600 Univ Ave, Toronto, ON M5G 1X5, Canada Mt Sinai Hosp 600 Univ Ave Toronto ON Canada M5G 1X5 1X5, Canada
Citazione:
P. Nash et al., "Multisite phosphorylation of a CDK inhibitor sets a threshold for the onset of DNA replication", NATURE, 414(6863), 2001, pp. 514-521

Abstract

SCF ubiquitin ligases target phosphorylated substrates for ubiquitin-dependent proteolysis by means of adapter subunits called F-box proteins. The F-box protein Cdc4 captures phosphorylated forms of the cyclin-dependent kinase inhibitor Sic1 for ubiquitination in late G1 phase, an event necessary for the onset of DNA replication. The WD40 repeat domain of Cdc4 binds with high affinity to a consensus phosphopeptide motif (the Cdc4 phospho-degron,CPD), yet Sic1 itself has many sub-optimal CPD motifs that act in concert to mediate Cdc4 binding. The weak CPD sites in Sic1 establish a phosphorylation threshold that delays degradation in vivo, and thereby establishes a minimal G1 phase period needed to ensure proper DNA replication. Multisite phosphorylation may be a more general mechanism to set thresholds in regulated protein-protein interactions.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 21:48:59