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Titolo:
Spontaneous reduction in Epstein-Barr virus (EBV) DNA copy number in EBV-infected epithelial cell lines
Autore:
Kanamori, M; Murakami, M; Takahashi, T; Kamada, N; Tajima, M; Okinaga, K; Miyazawa, Y; Kurata, T; Sairenji, T;
Indirizzi:
Tottori Univ, Fac Med, Sch Life Sci, Dept Biosignaling, Yonago, Tottori 6838503, Japan Tottori Univ Yonago Tottori Japan 6838503 Yonago, Tottori 6838503, Japan Tokyo Med & Dent Univ, Med Res Inst, Dept Tumor Virol, Div Virol & Immunol, Tokyo 1138510, Japan Tokyo Med & Dent Univ Tokyo Japan 1138510 Immunol, Tokyo 1138510, Japan Hiroshima Univ, Dept Canc Cytogenet, Res Inst Radiat Biol & Med, Hiroshima7340037, Japan Hiroshima Univ Hiroshima Japan 7340037 ol & Med, Hiroshima7340037, Japan Teikyo Univ, Sch Med, Cent Clin Lab, Tokyo 1730003, Japan Teikyo Univ Tokyo Japan 1730003 Med, Cent Clin Lab, Tokyo 1730003, Japan Teikyo Univ, Sch Med, Dept Surg, Tokyo 1730003, Japan Teikyo Univ Tokyo Japan 1730003 Sch Med, Dept Surg, Tokyo 1730003, Japan Teikyo Univ, Sch Med, Dept Clin Pathol, Tokyo 1730003, Japan Teikyo Univ Tokyo Japan 1730003 , Dept Clin Pathol, Tokyo 1730003, Japan Natl Inst Infect Dis, Dept Pathol, Tokyo 1620052, Japan Natl Inst Infect Dis Tokyo Japan 1620052 pt Pathol, Tokyo 1620052, Japan
Titolo Testata:
MICROBES AND INFECTION
fascicolo: 13, volume: 3, anno: 2001,
pagine: 1085 - 1091
SICI:
1286-4579(200111)3:13<1085:SRIEV(>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
BURKITTS-LYMPHOMA CELLS; NASOPHARYNGEAL CARCINOMA; GASTRIC-CARCINOMA; ESTABLISHMENT; REACTIVATION; INTEGRATION; CLONES; GROWTH; HNE-1; GENE;
Keywords:
EBV; EBV DNA copy; EBV in epithelial cells;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Sairenji, T Tottori Univ, Fac Med, Sch Life Sci, Dept Biosignaling, Yonago, Tottori 6838503, Japan Tottori Univ Yonago Tottori Japan 6838503 tori 6838503, Japan
Citazione:
M. Kanamori et al., "Spontaneous reduction in Epstein-Barr virus (EBV) DNA copy number in EBV-infected epithelial cell lines", MICROBES IN, 3(13), 2001, pp. 1085-1091

Abstract

We found that spontaneous and 12-0-tetradecanoylphorbol-13-acetate-inducedEpstein-Barr virus (EBV) reactivation occurred in short-term (ST)-culturedEBV-infected epithelial cell lines GT38 and GT39 after their establishment; however, it diminished in the long-term (LT)-cultured cells passaged for more than 2 years from ST-cultured cells. We hypothesized that the EBV reactivation may be related to the EBV DNA copy number in the cells. A higher level of EBV DNA content was detected in ST-cultured cells than in LT-cultured cells by Southern hybridization using an EBV DNA XhoI probe. Fluorescence in situ hybridization using EBV DNA BamHI W fragments showed that ST-cultured cells contained a higher EBV DNA copy number than that of LT-cultured cells. EBV DNA-negative cells were detected in small proportions in IT-cultured cells, but were undetected in ST-cultured cells. These results demonstrate that EBV genomes are not maintained stably in the cell lines, and someof them are lost in continuous passages of the cells. We discuss the mechanisms of reduction of EBV reactivation and EBV DNA in the cell lines. (C) 2001 Editions scientifiques et medicales Elsevier SAS.

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Documento generato il 11/07/20 alle ore 04:06:17