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Titolo:
Pathogenesis of mousepox in H-2(d) mice: evidence for MHC class I-restricted CD8(+) and MHC class II-restricted CD4(+) CTL antiviral activity in the lymph nodes, spleen and skin, but not in the conjunctivae
Autore:
Cespedes, IS; Toka, FN; Schollenberger, A; Gierynska, M; Niemialtowski, M;
Indirizzi:
Agr Univ Warsaw, Fac Vet Med, Div Virol Mycol & Immunol, SGGW, PL-03849 Warsaw, Poland Agr Univ Warsaw Warsaw Poland PL-03849 ol, SGGW, PL-03849 Warsaw, Poland
Titolo Testata:
MICROBES AND INFECTION
fascicolo: 13, volume: 3, anno: 2001,
pagine: 1063 - 1072
SICI:
1286-4579(200111)3:13<1063:POMIHM>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
HERPES-SIMPLEX VIRUS; T-LYMPHOCYTES; IMMUNE EVASION; VACCINIA VIRUS; FAS-LIGAND; CELLS; RESPONSES; INFECTION; PRIVILEGE; APOPTOSIS;
Keywords:
ectromelia (mousepox) virus; virus-specific CTL; pathogenesis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Niemialtowski, M Agr Univ Warsaw, Fac Vet Med, Div Virol Mycol & Immunol, SGGW, Grochowska 272, PL-03849 Warsaw, Poland Agr Univ Warsaw Grochowska 272 Warsaw Poland PL-03849 nd
Citazione:
I.S. Cespedes et al., "Pathogenesis of mousepox in H-2(d) mice: evidence for MHC class I-restricted CD8(+) and MHC class II-restricted CD4(+) CTL antiviral activity in the lymph nodes, spleen and skin, but not in the conjunctivae", MICROBES IN, 3(13), 2001, pp. 1063-1072

Abstract

Genetically sensitive mice (i.e. H-2(d) haplotype) infected with a naturalmouse pathogen named ectromelia virus (EV) can develop a mousepox. Virus replicates well in the skin, next in the draining lymph nodes (DLNs) and then in the spleen and liver, where it may induce extensive necrosis with strong inflammatory reaction. It is well known from the studies defined on someother viruses that a correlation, functional link and powerful help exist between MHC class I-restricted CD8(+) and MHC class II-restricted CD4(+) virus-specific cytotoxic T lymphocytes (CTLs). However, in the case of mousepox the role of CD4(+) CTLs is still controversial and some reports support the notion that induction of EV-specific CD4(+) CTLs is nonessential for the generation of virus-specific immune response. Consequently, this study was designed to evaluate Ev-specific CD8(+) and CD4(+) CTL activity in the DLNs, spleen, skin and conjunctivae of BALB/c (H-2(d)) mice at 7 and 14 days p.i. with Moscow strain of EV. By using bulk cytotoxicity assay and immunosurgery of effector T cells with mAb specific for CD4(+) and/or CD8(+) T cells our data show that EV-specific CD8(+) CTLs predominated in DLNs and spleen at 7 days (67 and 66% of total CTLs, respectively) and 14 days p.i. (63 and 69% of total CTLs, respectively). In contrast, we found that EV clearance from the cutaneous lesions during mousepox is CD4(+) CTL-dependent at 7 days p.i. (59%, of total CTLs), whereas at 14 days p.i. CD8(+) CTLs predominated in the epidermis, accounting for 72%, of the total EV-specific CTLs. Our studies showed that the population of EV-specific CTLs is heterogenous and contains cells of both phenotypes: CD8(+) and CD4(+). However, these effector cells did not express a similar tendency in cytotoxic activity in the DLNs, spleen and skin in comparison to the conjunctivae where EV-specificCD8(+) and CD4(+) CTLs were not detected at 7 days p.i. and at peak of mousepox conjunctivitis (14 days p.i.). Our results are discussed in terms of the value of EV to study antiviral CTL responses in the genetically susceptible host. (C) 2001 Editions scientifiques et medicales Elsevier SAS.

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Documento generato il 29/11/20 alle ore 09:27:29