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Titolo:
Mechanism of antifungal action of kanosamine
Autore:
Janiak, AM; Milewski, S;
Indirizzi:
Gdansk Tech Univ, Dept Pharmaceut Technol & Biochem, PL-80952 Gdansk, Poland Gdansk Tech Univ Gdansk Poland PL-80952 Biochem, PL-80952 Gdansk, Poland
Titolo Testata:
MEDICAL MYCOLOGY
fascicolo: 5, volume: 39, anno: 2001,
pagine: 401 - 408
SICI:
1369-3786(200110)39:5<401:MOAAOK>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
CANDIDA-ALBICANS; BINDING; GROWTH; MODE;
Keywords:
Candida albicans; chitin; enzyme inhibition; kanosamine;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Milewski, S Gdansk Tech Univ, Dept Pharmaceut Technol & Biochem, 11-12 Narutowicza St,PL-80952 Gdansk, Poland Gdansk Tech Univ 11-12 Narutowicza St Gdansk Poland PL-80952
Citazione:
A.M. Janiak e S. Milewski, "Mechanism of antifungal action of kanosamine", MED MYCOL, 39(5), 2001, pp. 401-408

Abstract

The antibiotic kanosamine inhibited growth of Saccharomyces cerevisiae anda range of human pathogenic fungi, including Candida albicans. Kanosamine was transported into C. albicans cells by the glucose transport system and subsequently phosphorylated. The product of its intracellular metabolism, kanosamine-6-phosphate, was an inhibitor of the enzyme glucosamine-6-phosphate synthase. Inhibition was competitive in respect to one of the substrates, D-fructose-6-phosphate, with K-i = 5.9 mM, and was non-competitive in respect to the second substrate, L-glutamine. On the other hand, kanosamine-6-phosphate had no effect on the enzyme catalysing the next metabolic step, namely glucosamine-6-phosphate N-acetylase. The action of kanosamine on C albicans cells resulted in profound morphological changes, inhibition of septum formation and cell agglutination. Experiments with S. cerevisiae mutantsshowed that the presence of the Cdr1p drug efflux pump did not affect the antifungal activity of kanosamine.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/03/20 alle ore 10:20:56