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Titolo:
Semi-quantitative RT-PCR analysis of environmental influence on P450scc and PNMT mRNA expression in rat adrenal glands
Autore:
Morita, K; Kuwada, A; Sano, A; Sakata, M; Sei, H; Morita, Y;
Indirizzi:
Univ Tokushima, Sch Med, Dept Pharmacol, Tokushima 7708503, Japan Univ Tokushima Tokushima Japan 7708503 armacol, Tokushima 7708503, Japan Univ Tokushima, Sch Med, Dept Physiol, Tokushima 7708503, Japan Univ Tokushima Tokushima Japan 7708503 Physiol, Tokushima 7708503, Japan
Titolo Testata:
LIFE SCIENCES
fascicolo: 1, volume: 70, anno: 2001,
pagine: 73 - 80
SICI:
0024-3205(20011121)70:1<73:SRAOEI>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
NERVE GROWTH-FACTOR; NEUROTROPHIC FACTOR EXPRESSION; ANTERODORSAL THALAMI NUCLEI; FACTOR MESSENGER-RNA; GENE-EXPRESSION; SPATIAL MEMORY; VISUAL-CORTEX; BRAIN-DAMAGE; AGED RATS; HIPPOCAMPUS;
Keywords:
semi-quantitative RT-PCR analysis; environmental conditions; p450scc mRNA; PNMT mRNA; adrenal function;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Morita, K Univ Tokushima, Sch Med, Dept Pharmacol, 3-18-15 Kuramoto, Tokushima 7708503, Japan Univ Tokushima 3-18-15 Kuramoto Tokushima Japan 7708503 , Japan
Citazione:
K. Morita et al., "Semi-quantitative RT-PCR analysis of environmental influence on P450scc and PNMT mRNA expression in rat adrenal glands", LIFE SCI, 70(1), 2001, pp. 73-80

Abstract

Environmental influence on brain function, particularly spatial learning and memory, has been extensively investigated, but little is known about theinfluence of environmental conditions on the functions of peripheral organs. In the present study, the effects of different housing conditions on thesteady-state levels of mRNAs encoding cholesterol side-chain cleavage enzyme (cytochrome P450scc) and phenylethanolamine N-methyltransferase (PNMT) in adrenal glands was examined to investigate the environmental influence onboth adrenocortical and adrenomedullary functions. Behavioral changes of the animals housed in different conditions were first examined to assess therelevance of environmental manipulation used. In consistent with previous findings, housing of the animals in enriched conditions resulted in the significant reduction of spontaneous motor activity (locomotor activity and rearing) in comparison with housing in isolated conditions, thus indicating the relevance of housing conditions used in this work for investigating the environmental influence on adrenal function. Then, the effects of these housing conditions on P450scc and PNMT mRNA levels in adrenal glands were examined using semi-quantitative RT-PCR method. In comparison with the isolatedgroup, the enriched group showed significantly higher levels of P450scc mRNA. In contrast, PNMT mRNA levels in the enriched group were significantly lower than those in the isolated group. These results propose the possibility that the environmental conditions may cause differential alterations in adrenocortical and adrenomedullary functions, although their possible association with behavioral changes still remains to be elucidated. (C) 2001 Elsevier Science Inc. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 02:19:32