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Titolo:
Platelet aggregation is not initiated by platelet shape change
Autore:
Maurer-Spurej, E; Devine, DV;
Indirizzi:
Univ British Columbia, Dept Pathol & Lab Med, Vancouver, BC V6T 2B5, Canada Univ British Columbia Vancouver BC Canada V6T 2B5 ver, BC V6T 2B5, Canada Canadian Blood Serv, Vancouver, BC, Canada Canadian Blood Serv Vancouver BC Canada lood Serv, Vancouver, BC, Canada
Titolo Testata:
LABORATORY INVESTIGATION
fascicolo: 11, volume: 81, anno: 2001,
pagine: 1517 - 1525
SICI:
0023-6837(200111)81:11<1517:PAINIB>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
BLOOD-PLATELETS; LIGHT-SCATTERING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Maurer-Spurej, E Univ British Columbia, Dept Pathol & Lab Med, 2211 Wesbrook Mall, Vancouver, BC V6T 2B5, Canada Univ British Columbia 2211 Wesbrook Mall Vancouver BC Canada V6T 2B5
Citazione:
E. Maurer-Spurej e D.V. Devine, "Platelet aggregation is not initiated by platelet shape change", LAB INV, 81(11), 2001, pp. 1517-1525

Abstract

Because the initial decrease in light transmission in platelet aggregometry is attributed to platelet shape change, it is widely held that platelet shape change is a prerequisite for platelet aggregation. We conducted this study to determine the basis of this initial optical effect in aggregometry. Platelets were activated with ADP, thrombin, or the thrombin receptor agonist peptide SFLLRN (TRAP(1-6)). In every case the initial decrease in lighttransmission occurred with the concomitant formation of microaggregates. This was also seen when preactivated platelets, which cannot undergo furthermorphological changes, were used, and when platelets were activated in thepresence of shape-change inhibitors such as cytochalasin D and vincristine. Microscopy analysis of samples fixed at minimum light transmission in theaggregometer, which is generally assumed to signal shape change, always showed the presence of microaggregates. Microaggregation appeared to be distinct from full aggregation, as it was not inhibited by the addition of CD61,an antibody to the beta (3) Integrin. To model these findings, fibrinogen-coated latex spheres, which cannot change shape, were aggregated with thrombin; the initial decrease in light transmission was still seen, and microaggregates formed at this time. These results indicate that platelet shape change Is not a prerequisite for aggregation and that the signal widely believed to represent shape change reflects platelet microaggregation instead. We conclude that platelet aggregation occurs independently of shape change and that shape change is not necessarily followed by aggregation. These observations suggest an alternative role for platelet shape change of single platelets.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/01/20 alle ore 10:34:40