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Titolo:
Induction of Hsp60 by photofrin-mediated photodynamic therapy
Autore:
Hanlon, JG; Adams, K; Rainbow, AJ; Gupta, RS; Singh, G;
Indirizzi:
Hamilton Reg Canc Ctr, Hamilton, ON L8V 5C2, Canada Hamilton Reg Canc CtrHamilton ON Canada L8V 5C2 lton, ON L8V 5C2, Canada McMaster Univ, Dept Biol, Hamilton, ON L8V 2C5, Canada McMaster Univ Hamilton ON Canada L8V 2C5 ol, Hamilton, ON L8V 2C5, Canada McMaster Univ, Dept Biochem, Hamilton, ON L8V 2C5, Canada McMaster Univ Hamilton ON Canada L8V 2C5 em, Hamilton, ON L8V 2C5, Canada McMaster Univ, Dept Pathol & Mol Med, Hamilton, ON L8V 2C5, Canada McMaster Univ Hamilton ON Canada L8V 2C5 ed, Hamilton, ON L8V 2C5, Canada
Titolo Testata:
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
fascicolo: 1, volume: 64, anno: 2001,
pagine: 55 - 61
SICI:
1011-1344(20011101)64:1<55:IOHBPP>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEAT-SHOCK PROTEINS; HUMAN-CELLS INVITRO; MURINE TUMOR-CELLS; OXIDATIVE STRESS; AMINOLEVULINIC-ACID; CROSS-RESISTANCE; MAMMALIAN-CELLS; HEMATOPORPHYRIN; EXPRESSION; PHOTOSENSITIZATION;
Keywords:
PDT; Photofrin II (R); heat shock proteins; Hsp60;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Singh, G Hamilton Reg Canc Ctr, 699 Concess St, Hamilton, ON L8V 5C2, Canada Hamilton Reg Canc Ctr 699 Concess St Hamilton ON Canada L8V 5C2 a
Citazione:
J.G. Hanlon et al., "Induction of Hsp60 by photofrin-mediated photodynamic therapy", J PHOTOCH B, 64(1), 2001, pp. 55-61

Abstract

Photodynamic therapy (PDT) invokes a number of cellular responses. Other studies have shown that PDT induces transcription and translation of heat shock proteins (Hsps). The expression of mitochondrial heat shock protein, Hsp60, was measured following in vitro Photofrin-mediated PDT in the colon cancer cell line HT29 and its PDT-induced resistant variant HT29-P14 as well as the radiation-induced fibrosarcoma cells RIF-I and its PDT-induced resistant variant, RIF-8A. Basal levels of Hsp60 were found to be similar in thetwo murine cell lines. In the human model, the resistant HT29-P14 cell line showed a small increase in basal levels relative to its parental population. Incubation with Photofrin (R) (PH) alone or photosensitization caused asignificant increase in Hsp60 levels in all cell lines as determined by flow cytometry. A dose-dependent and temporal relationship for PDT response was observed, maximum levels were detected 6-8 h post PDT, at which time, Hsp60 induction was found to be significantly greater in the two resistant variants. Induction in the RIF cells was also found to be greater after incubation with PH alone at the highest doses tested. These results indicate that the presence of PH and the subsequent oxidative stress of PDT can induce Hsp60 and implicated it as a common factor that may contribute to the resistance observed in the induced resistant cells. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/09/20 alle ore 22:11:27