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Titolo:
The relationship between UVB screening and cytoprotection by microcorpuscular ZnO or ascorbate against DNA photodamage and membrane injuries in keratinocytes by oxidative stress
Autore:
Hayashi, S; Takeshita, H; Nagao, N; Nikaido, O; Miwa, N;
Indirizzi:
Hiroshima Prefectural Univ, Sch Biosci, Div Cell Biochem, Hiroshima 7270023, Japan Hiroshima Prefectural Univ Hiroshima Japan 7270023 oshima 7270023, Japan Kanazawa Univ, Fac Pharmaceut Sci, Div Radiat Biol, Kanazawa, Ishikawa 920, Japan Kanazawa Univ Kanazawa Ishikawa Japan 920 , Kanazawa, Ishikawa 920, Japan
Titolo Testata:
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
fascicolo: 1, volume: 64, anno: 2001,
pagine: 27 - 35
SICI:
1011-1344(20011101)64:1<27:TRBUSA>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN SKIN FIBROBLASTS; MICROFINE ZINC-OXIDE; TITANIUM-DIOXIDE; IN-VITRO; ULTRAVIOLET; PROTECTION; SUNSCREENS; CELLS; CYTOTOXICITY; PEROXIDATION;
Keywords:
zinc oxide; ascorbic acid; UVB-injuries; keratinocytes; cell mortality; cell membrane integrity disruption;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Miwa, N Hiroshima Prefectural Univ, Sch Biosci, Div Cell Biochem, Hiroshima 7270023, Japan Hiroshima Prefectural Univ Hiroshima Japan 7270023 270023, Japan
Citazione:
S. Hayashi et al., "The relationship between UVB screening and cytoprotection by microcorpuscular ZnO or ascorbate against DNA photodamage and membrane injuries in keratinocytes by oxidative stress", J PHOTOCH B, 64(1), 2001, pp. 27-35

Abstract

Decreased cell viability and increased formation of cyclobutane-type pyrimidine dimers (CPDs) in DNA of UVB-irradiated keratinocytes were shown to beappreciably restored by the addition of w/o emulsion of microcorpuscular zinc oxide (mcZnO) with a corpuscle diameter of 0.15 mum. The cytoprotectionwas exerted only by 20 wt/wt% mcZnO at levels equivalent to 40- to 100-mum-thick emulsion layers, which screened 90-92% of the incident UVB. However,protection was not seen by mcZnO below 20-mum thickness, which, unexpectedly, screened 79% of the incident radiation. This suggests that thorough UVBscreening is necessary for cytoprotection. This may be attributable to involvement of intracellular reactive oxygen species (ROS) secondarily generated from UVB-irradiated mcZnO. Intracellular ROS was increased in mcZnO-added cells in a time-dependent manner even after UVB irradiation, contrasting with reduction of intracellular ROS in ascorbic acid-added cells. UVB-induced disruption of cell membrane integrity was reduced by mcZnO at 100-mum thickness, equivalent to the addition of ascorbic acid of 50 muM. Thus, mcZnOwas thought to be cytoprotective through reductions of intracellular ROS generation, CPD formation and cell membrane disintegration when added so abundantly so as to achieve UVB-screening more than 90%. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 29/09/20 alle ore 20:28:22