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Titolo:
A validated HPLC method for the determination of pyridostigmine bromide, acetaminophen, acetylsalicylic acid and caffeine in rat plasma and urine
Autore:
Abu-Qare, AW; Abou-Donia, MB;
Indirizzi:
Duke Univ, Med Ctr, Dept Pharmacol & Canc Biol, Durham, NC 27710 USA Duke Univ Durham NC USA 27710 Pharmacol & Canc Biol, Durham, NC 27710 USA
Titolo Testata:
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
fascicolo: 5-6, volume: 26, anno: 2001,
pagine: 939 - 947
SICI:
0731-7085(200112)26:5-6<939:AVHMFT>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
PERFORMANCE LIQUID-CHROMATOGRAPHY; BIOLOGICAL-FLUIDS; SALICYLIC-ACID; METABOLITES; PARACETAMOL; ASPIRIN; ASSAY; PHARMACOKINETICS; DEET;
Keywords:
pyridostigmine bromides; acetylsalicylic acid; acetaminophen; caffeine;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Abou-Donia, MB Duke Univ, Med Ctr, Dept Pharmacol & Canc Biol, POB 3813, Durham, NC 27710USA Duke Univ POB 3813 Durham NC USA 27710 Durham, NC 27710USA
Citazione:
A.W. Abu-Qare e M.B. Abou-Donia, "A validated HPLC method for the determination of pyridostigmine bromide, acetaminophen, acetylsalicylic acid and caffeine in rat plasma and urine", J PHARM B, 26(5-6), 2001, pp. 939-947

Abstract

A method was developed for the separation and quantification of the anti-nerve agent pyridostgmine bromide (PB;3-dimethylaminocarbonytoxy-N-methyl pyridinium bromide), the analgesic drugs acetaminophen and acetylsalicylic acid. and the stimulant caffeine (3,7-dihydro-1,3,7-trimethyl-1 H-purine-2,6-dione) in rat plasma and urine. The compounds were extracted using C-18 Sep-Pak(R) cartridges then analyzed by high performance liquid chromatography (HPLC) with reversed phase C-18 column, and UV detection at 280 nm. The compounds were separated using gradient of 1-85% acetonitrile in water (pH 3.0) at a flow rate ranging between 1 and 1.5 ml/min in a period of 14 min. The retention times ranged from 8.8 to 11.5 min, The limits of detection wereranged between 100 and 200 ng/ml, while limits of quantitation were 150-200 ng/ml. Average percentage recovery of five spiked plasma samples were 70.9 +/- 9.5, 73.7 +/- 9.8, 88.6 +/- 9.3, 83.9 +/- 7.8, and from urine 69.1 +/- 8.5, 74.5 +/- 8.7, 85.9 +/- 9.8, 83.2 +/- 9.3, for pyridostigmine bromide, acetaminophen, acetylsalicylic acid and caffeine, respectively. The relationship between peak areas and concentration was linear over range between 100 and 1000 ng/ml. The resulting chromatograms showed no interfering peaksfrom endogenous plasma or urine components. This method was applied to analyze these compounds following oral administration in rats. (C) 2001 Elsevier Science B.V, All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/07/20 alle ore 03:35:01